Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1141740 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Macrophages mediate key antimicrobial responses against intracellular bacterial pathogens, such as Salmonella enterica. Yet, they can also act as a permissive niche for these pathogens to persist in infected tissues within granulomas, which are immunological structures comprised of macrophages and other immune cells. We apply single-cell transcriptomics to investigate macrophage functional diversity during persistent Salmonella enterica serovar Typhimurium (STm) infection in mice. We identify determinants of macrophage heterogeneity in infected spleens and describe populations of distinct phenotypes, functional programming, and spatial localization. Using a STm mutant with impaired ability to polarize macrophage phenotypes, we find that angiotensin converting enzyme (ACE) defines a granuloma macrophage population that is non-permissive for intracellular bacteria and their abundance anticorrelates with tissue bacterial burden. Disruption of pathogen control by neutralizing TNF is linked to preferential depletion of ACE+ macrophages in infected tissues. Thus ACE+ macrophages have limited capacity to serve as cellular niche for intracellular bacteria to establish persistent infection.
2022-10-20 | GSE215880 | GEO
Project description:Analysis of gene expression profiles of 12 normal mice infected with Salmonella using deep sequencing technology.
2020-08-13 | GSE115164 | GEO
Project description:To have a global picture of the miRNAs regulated upon treatement with secretome of Salmonella infected cells, we assessed small RNA changes, by RNA-sequencing, of HeLa cells treated with sectretome of Salmonella infected cells or mock-treated cells
2021-04-12 | GSE147363 | GEO
Project description:Analysis of differential genome-wide gene expression within the splenic homogenate of A/J and AcB60 mice, both naïve mice and those infected with Salmonella Typhimrium Total RNA was islolated from the splenic homogenate of naïve A/J and AcB60 mice, as well as mice infected with 1000 CFUs of Salmonella Typhimuirum and sacrified 3, 5, and 7 days post-infection
2015-02-12 | E-GEOD-65893 | biostudies-arrayexpress
Project description:Analysis of differential genome-wide gene expression within the splenic homogenate of A/J and AcB60 mice, both naïve mice and those infected with Salmonella Typhimrium
2015-02-12 | GSE65893 | GEO
Project description:Quantitative proteomics of GAS infected human blood and Salmonella infected mouse spleens
2018-01-30 | MSV000081997 | MassIVE
Project description:Cecal content of mice infected with Salmonella run on Q-Exactive. Reversed-phase, ESI positive, polar C18 column (2.6 um particle size, 2.1 mm x 100 mm Phenomenex, Kinetex).
2024-08-20 | MSV000095657 | GNPS
Project description:Objective: Reg3g has been proposed to have a protective role against infection due to its bactericidal effect on Gram-positive bacteria, but evidence from in vivo studies is lacking. Therefore we generated a Reg3g-/- mouse, to determine its role in intestinal homeostasis and protection against experimental infection. Methods: Reg3g-/- mice were phenotyped using histological methods and a range of innate and immune markers. To investigate the antimicrobial role of Reg3g we experimentally infected mice with Gram-positive Listeria monocytogenes and Gram-negative Salmonella entertitidis and measured translocated bacteria, mucosal and systemic markers of infection. Results: Reg3g-/- mice display altered ileal mucus distribution and increased bacterial contact with the epithelium. , concomitant with This increased the inflammatory status in of the ileal mucosa and increased expression of Il-22, myeloperoxidase (MPO) and serum chemokines in serum. In response to infection, Reg3g-/- mice showed transcriptome changes and elevated levels of mucosal MPO in the ileum, but no increased bacterial translocation to the organs. Conclusions: Reg3g is equally distributed throughout the mucus of wild type (wt) mice and its absence results in an altered distribution of the ileal mucus. Reg3g deficiency also results resulted in increased bacterial contact with the epithelium and heightened inflammatory responses in the ileal mucosa. We propose that Reg3g binds pathogens suggesting it and contributes to mucus barrier function by ensnaring bacteria. Compared to wt mice, Reg3g-/- mice infected with S. enteritidis and L. monocytogenes show an increase of mucosal inflammatory markers indicating the protective, anti-microbial roles of Reg3g in defence against both Gram-positive and -negative bacteria. This study was set up according to a one-treatment, one-control design; treatments were inoculation with either Listeria monocytogenes or Salmonella enteritidis bacterial pathogens. The study results contain transcriptional profiles from infected and sham-infected control C57Bl/6 mice. In total, this study includes data from 2 treatments and 1 control of (pooled) wild-type C57Bl/6 mice and Reg3g-/- KO mutant C57Bl/6 mice = 6 arrays.
2014-06-06 | E-GEOD-44409 | biostudies-arrayexpress