Project description:We performed single cell RNA sequencing to investigate the gene expression changes upon maternal stress in progeny drosophila posterior midgut.
Project description:We performed single cell RNA sequencing to investigate the gene expression changes upon maternal stress in progeny drosophila posterior midgut.
Project description:We found that the midgut shows striking regional differentiation along its anterior-posterior axis. Ten distinct subregions differ in cell morphology, gene expression and aspects of Notch signaling. RNA from isolated regions that was analyzed by RNAseq revealed spatially regulated expression of hundreds of enzymes and other genes with likely tissue functions. 10 midgut segments comprising from 1-3 subregions x 3 replicates from each segment = 30 samples
Project description:We found that the midgut shows striking regional differentiation along its anterior-posterior axis. Ten distinct subregions differ in cell morphology, gene expression and aspects of Notch signaling. RNA from isolated regions that was analyzed by RNAseq revealed spatially regulated expression of hundreds of enzymes and other genes with likely tissue functions.
Project description:Methionine restriction is known to extend lifespan in various model organisms including Drosophila melanogaster. In this analysis, we performed scRNAseq of Drosophila female midgut samples to understand the cell type specific response to methionine restriction.
Project description:We mapped about 25 million reads per sample to the Drosophila melanogaster genome (dm6). We confirmed that sterol processing genes are selectively expressed in the posterior midgut. Modifying sterol metabolism in the diet or with Hr96 mutants resulted in significant changes in metabolism transcripts, hormones expressed in enteroendocrine cells, and Notch pathway related genes.
Project description:Comparisons between EcR mutant midgut and a reference control sample from mixed-stage normal midgut Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development Keywords: other
Project description:A midgut reference sample is compared to midgut samples taken at various stages before (18h, 4h), at (0h), and after (2h, 3h, 4h, 5h, 6h, 8h, 10h, 12h) puparium formation. Keywords = Drosophila, ecdysone, network, genomic, microarray, organogenesis, EcR, midgut, central nervous system, salivary gland, epidermis, imaginal disc, development Keywords: other
Project description:Mosquito midgut is the first tissue encounter the virus. We apply single cell RNA sequencing to investigate the gene difference in cell level between blood fed and Zika virus infected midgut.