Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Soil fungi in topsoil and subsoil of a temperate agrosilvopastoral and syntropic agroforestry system

PROVIDER: PRJNA1142770 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30068000_1.fastq.gz	Fastqsanger.gz
	SRR30068000_2.fastq.gz	Fastqsanger.gz
	SRR30068001_1.fastq.gz	Fastqsanger.gz
	SRR30068001_2.fastq.gz	Fastqsanger.gz
	SRR30067906_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 192

Similar Datasets

Project description:Soil bacteria in topsoil and subsoil of a temperate agrosilvopastoral and syntropic agroforestry system

| PRJNA1142703 | ENA

Project description:Soil fungi in a temperate agroforestry system

| PRJNA885015 | ENA

Project description:Study on soil fungi in different soil layers of agroforestry system

| PRJNA1108612 | ENA

Project description:Topsoil fungi

| PRJNA1206529 | ENA

Project description:Plant-nitrifier interactions in topsoil and subsoil

| PRJNA1080623 | ENA

Project description:Soil fungal communities in temperate agroforestry cropland systems

| PRJNA667608 | ENA

Subsoil_AMF

Project description:Subsoil AMF within a temperate agricultural field

| PRJEB54814 | ENA

Project description:soil fungal communities in a young agroforestry system

| PRJNA715353 | ENA

Early life exposure of pigs to topsoil alters miRNA and mRNA expression in peripheral blood mononuclear cells

Project description:Exposure to less hygienic conditions during early childhood has been associated with stimulation and development of the immune system. A recent study indicated exposure of piglets to soil-borne microbes during lactation was related with modulation of gut microbiota and immune function. To identify potential molecular mechanisms and pathways impacted by early life topsoil exposure, we analyzed gene expression (mRNA and miRNA) in peripheral blood mononuclear cells (PBMC) from these piglets. Total RNA was extracted from PBMC of piglets exposed to topsoil only from d 4-d 21 of life (mRNA n=6; miRNA n=5) or unexposed control pigs (mRNA n=6; miRNA n=8) at 11, 20, and 56 days of age. Small RNA and mRNA were sequenced with 50-bp single-end reads using Illumina chemistry. Differential expression (DE) analysis was performed using PROC Glimmix of SAS to evaluate changes in expression due to topsoil exposure over time with genes declared DE at a false discovery rate (FDR) of q<0.10. A total of 138 mRNA and 21 miRNAs were identified as DE for the treatment by age interaction. Ontology enrichment analysis of DE mRNA revealed GO terms directly involved in the connection between T-cell and antigen-presenting cells that are associated with T-cell activation. Key regulatory genes identified include PTPRJ, ITGB3, TRBV30, CD3D, mir-143, mir-29, and mir-148a. While these results require validation, this study provides data supporting the hypothesis that less hygienic environments during early life may contribute to the development of the immune system

2022-07-31 | GSE205321 | GEO

Project description:Tree rhizosphere soil fungi in temperate forest

| PRJNA834583 | ENA