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Developmentally regulated generation of a systemic signal for long-lasting defence priming in tomato [small RNA-seq]

ABSTRACT: Developmentally regulated generation of a systemic signal for long-lasting defence priming in tomato [small RNA-seq]

PROVIDER: PRJNA1144134 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30126603.fastq.gz	Fastqsanger.gz
	SRR30126604.fastq.gz	Fastqsanger.gz
	SRR30126605.fastq.gz	Fastqsanger.gz
	SRR30126606.fastq.gz	Fastqsanger.gz
	SRR30126607.fastq.gz	Fastqsanger.gz

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Developmentally regulated generation of a systemic signal for long-lasting defence priming in tomato [WGBS]

Project description:Developmentally regulated generation of a systemic signal for long-lasting defence priming in tomato [WGBS]

| PRJNA1144133 | ENA

Developmentally regulated generation of a systemic signal for long-lasting defence priming in tomato [RNA-seq]

Project description:Developmentally regulated generation of a systemic signal for long-lasting defence priming in tomato [RNA-seq]

| PRJNA1144135 | ENA

Developmentally regulated generation of a systemic signal for long-lasting defence priming in tomato [WGBS]

Project description:Tomato is a major global crop however its production is limited by Botrytis cinerea. Due to the toxicity of post-harvest pesticide application, alternative control methods such as priming are being investigated. Plants were treated with β-aminobutyric (BABA) at two developmental stages and resistance against B. cinerea was tested in fruit tissue and in progenies. DNA methylation and RNA sequencing were conducted to characterise the (epi)genetic changes associated with long-lasting resistance. Grafting experiments were done to assess the systemic nature of this signal, which was further characterised by small RNA (sRNA) sequencing of scions. Only BABA-treated seedlings displayed induced resistance (IR). DNA methylation analysis revealed seedling-specific changes, which occurred in the context of lower basal methylation. BABA-IR was found to be transmissible from primed rootstock to grafted unprimed scions. In these scions, we identified a subset of mobile 24 nt sRNAs associated with primed genes to infection in fruit. Our results demonstrate the functional association of a systemic signal with long lasting IR and priming. Through integrated omics approaches we have identified markers of long-lasting priming in tomato fruit which could also serve as targets for durable resistance in other crops.

2024-11-17 | GSE273942 | GEO

Developmentally regulated generation of a systemic signal for long-lasting defence priming in tomato [RNA-seq]

2024-11-17 | GSE273944 | GEO

Developmentally regulated generation of a systemic signal for long-lasting defence priming in tomato [small RNA-seq]

2024-11-17 | GSE273943 | GEO

Long-lasting priming by β-amino butyric acid is marked by DNA hypomethylation

Project description:Exposure of plants to stress conditions or to certain chemical elicitors can establish a primed state, whereby responses to future stress encounters are enhanced. Stress priming can be long-lasting and likely involves epigenetic regulation of stress-responsive gene expression. However, the molecular events underlying priming are not well understood. Here, we characterise epigenetic changes in tomato plants primed for pathogen resistance by treatment with β-aminobutyric acid (BABA). We used whole genome bisulphite sequencing to construct tomato methylomes from control plants and plants treated with BABA at the seedling stage, and a parallel transcriptome analysis to identify genes primed for the response to inoculation by the fungal pathogen, Botrytis cinerea. Genomes of plants treated with BABA showed a significant reduction in global cytosine methylation, especially in CHH sequence contexts. Analysis of differentially methylated regions (DMRs) revealed that CHH DMRs were almost exclusively hypomethylated and were enriched in gene promoters and in DNA transposons located in the chromosome arms. Although there was some enrichment of stress response-related gene ontology terms in genes overlapping CHH DMRs, there was no significant enrichment of DMRs amongst a set of 747 primed defence genes, and nor was the pattern of methylation in these genes altered by BABA treatment. Hence, we conclude that whilst BABA treatment of tomato seedlings results in characteristic changes in genome-wide DNA methylation, CHH hypomethylation does not appear to directly target genes showing primed responses to pathogen infection. Instead, methylation may confer priming via in-trans regulation, acting at a distance from defence genes, and/or by targeting a smaller group of regulatory genes controlling stress responses.

2022-04-05 | GSE190780 | GEO

Long-lasting priming by β-amino butyric acid is marked by DNA hypomethylation

Project description:Long-lasting priming by β-amino butyric acid is marked by DNA hypomethylation

| PRJNA788469 | ENA

Long-lasting priming by β-amino butyric acid is marked by DNA hypomethylation.

Project description:Long-lasting priming by β-amino butyric acid is marked by DNA hypomethylation.

| PRJNA788467 | ENA

Chitosan primes plant defence mechanisms against Botrytis cinerea including Avr9/Cf-9 rapidly-elicited genes

Project description:Current protection strategies against the fungal pathogen Botrytis cinerea rely on a combination of conventional fungicides and host genetic resistance. Defence elicitors can stimulate plant defence mechanisms through a phenomenon known as priming. Priming results on a faster and/or stronger expression of resistance upon pathogen attack. This work aims to study priming of a commercial formulation of the elicitor Chitosan. Treatments with Chitosan result in induced resistance in solanaceous and brassicaceous plants. Large-scale transcriptomic analysis in this study revealed that Chitosan primes gene expression at early time-points after infection. Four conditions were analysed using microarrays: (i) water-treated and non-infected plants (Water + Mock); (ii) Chitosan-treated and non-infected plants (Chitosan + Mock); (iii) water-treated and B. cinerea-infected plants (Water + B. cinerea); (iv) Chitosan-treated and B. cinerea-infected plants (Chitosan + B. cinerea). Inoculations were performed four days after treatment with Chitosan, and leaf discs from four independent plants (biological replicates) per treatment were sampled at 6 h, 9 h and 12 h post-inoculation (hpi) with water mock or B. cinerea spores.

2020-07-01 | E-MTAB-8868 | biostudies-arrayexpress

Priming resistance of tomato to Botrytis cinerea infection by root drench treatment with beta-aminobutyric acid.

Project description:Induced resistance responses of plants to biotic stress can be enhanced by prior treatment by chemical priming agents such as beta-aminobutyric acid (BABA). Defence-related genes are typically not expressed following priming, but show faster and/or stronger induced expression following infection. Here we test the transcriptome responses of control and BABA-primed plants to infection with the fungal pathogen Botrytis cinerea.

2022-04-05 | GSE190779 | GEO

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