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Effect of APC depletion on gene expression in JEG-3 cells

ABSTRACT: Effect of APC depletion on gene expression in JEG-3 cells

PROVIDER: PRJNA1144136 | ENA |

REPOSITORIES: ENA

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Effect of APC depletion on gene expression in JEG-3 cells

Project description:Fetal-maternal immune tolerance guarantees a successful pregnancy throughout gestation. HLA-G, a non-classical human leukocyte antigen (HLA) molecule exclusively expressed in extravillous trophoblasts (EVT), is a crucial factor in establishing fetal-maternal immune tolerance by interacting with inhibitory receptors on various maternal immune cells residing in the uterus. While trophoblast specific Cis-Regulatory Elements (CREs) impacting HLA-G transcription have been described, the identity of trans-acting factors controlling HLA-G expression in EVT remains poorly understood. Utilizing a genome-wide CRISPR-Cas9 knockout screen, we find that the WNT signaling pathway negatively regulates HLA-G expression in EVT. APC is a key component of the destruction complex for cytoplasmic β-catenin, switching off the canonical WNT pathway. This dataset containes the transcriptomics in JEG-3 cells upon APC knockout.

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Effect of ELF3 and NLRP7 depletion on gene expression in JEG-3 cells

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Effect of IL-11 on gene expression in JEG-3 and HTR-8/SVneo trophoblastic cells

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Effect of LIF and IL-6 on gene expression in JEG-3 and HTR-8/SVneo trophoblastic cells

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Effect of IL-11 on gene expression in JEG-3 and HTR-8/SVneo trophoblastic cells

Project description:IL-11 increases the invasiveness of JEG-3 cells while, reduces the invasiveness of HTR-8/SVneo cells. This study examines the effect of IL-11 on gene expression in trophobalstic cell models viz. JEG-3 and HTR-8/SVneo cells to resolve the controversies associated with the IL-11 mediated regulation of the invasiveness of these two cell lines. JEG-3 and HTR-8/Svneo cells were stimulated with IL-11 (200 ng/ml) for 24 h in pain medium keeping un-treated cells as control. After 24 h of stimulation, total RNA was isolated from these cells and used for the microarray experiments. These experiments were performed once.

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Effect of LIF and IL-6 on gene expression in JEG-3 and HTR-8/SVneo trophoblastic cells

Project description:Both leukemia inhibitory factor (LIF) and interleukin-6 (IL-6) increase the invasiveness of JEG-3 and HTR-8/SVneo cells. This study examines the effect of LIF and IL-6 on gene expression in trophoblastic cell models viz. JEG-3 and HTR-8/SVneo cells to decipher the molecular basis of the increase in invasiveness. JEG-3 and HTR-8/Svneo cells were stimulated with LIF (50 ng/ml) and IL-6 (100 ng/ml) for 24 h in plain medium keeping untreated cells as a control. After 24 h of stimulation, total RNA was isolated from these cells and used for microarray experiments. These experiments were performed once.

2012-05-31 | E-GEOD-36202 | biostudies-arrayexpress

Effect of WNT hyperactivation by CTNNB1 overexpression or APC knockdown on gene expression in HT29 cells

Project description:To investigate effect of WNT hyperactivation by CTNNB1 overexpression or APC knockdown on gene expression and probe possible mechanism of cell viability effect, we overexpressed GFP/CTNNB1, or induce knockdown of APC by shNT1 (non-targeting) and shAPC

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Transcription profiling by array of Apc-deficient mouse small intestinal epithelium upon Msx1 depletion.

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Effect of depletion of Elovl6 on gene expression

Project description:Effect of depletion of Elovl6 on gene expression

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Transcription profiling by array of Apc-deficient proximal colonic epithelium upon Msx1 transcription factor depletion

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