Project description:The objective of this study was to determine gene expression changes that occur as a result of Ndrg3-deficiency in mature CD8+ thymocytes. Our research has revealed that conditional ablation of Ndrg3 in the T cell lineage causes a significant loss of naive CD8+ T cells, and in order to determine the basis for this defect, we isolated mature, CD8 single-positive thymocytes from control and knockout mice, and subjected them to RNA-seq analysis to identify genes that were dysregulated in the absence of Ndrg3.
Project description:Comparing the mRNA expression profiles of c-Myb deficient and c-Myb sufficient Tcra-/- DP thymocytes. Results provide insight into the role of c-Myb in the regulation of survival and differentiation during the pre-selection DP stage where c-Myb expression is abundant during T cell development. DP thymocytes were purified from four c-Myb deficient and four c-Myb sufficient mice over magnetic columns. RNA from each biological replicate was individually hybridized onto a total of eight MOE430 2.0 Chips.