Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [RNA-seq]

ABSTRACT: RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [RNA-seq]

PROVIDER: PRJNA1145380 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30163230_1.fastq.gz	Fastqsanger.gz
	SRR30163230_2.fastq.gz	Fastqsanger.gz
	SRR30163231_1.fastq.gz	Fastqsanger.gz
	SRR30163231_2.fastq.gz	Fastqsanger.gz
	SRR30163232_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [RNA-seq]

Project description:The centromere is a pivotal chromatin domain that ensures accurate chromosome segregation during cell division. However, the epigenome regulation of the centromere and its impact on centromere function remain largely elusive. Here in the model plant Arabidopsis, we show that CCR4, the catalytic subunit of the RNA deadenylation complex CCR4-NOT, is essential for maintenance of the centromere epigenome and chromosome integrity. We demonstrate that CCR4 is involved in shortening of the poly(A) tails of transcripts originated from centromeric transposons and repeats, thereby promoting the production of small interfering RNAs (siRNAs). The CCR4-dependent siRNAs guide non-CG DNA methylation at centromere repeats, and CCR4 cooperates with canonical DNA methylation pathways to enhance centromeric H3K9 methylation and ensure mitotic chromosome stability. Our study illustrates the crucial role of RNA quality control in RNA interference and reveals the elaborate mechanism that safeguards plant centromeres through epigenomic regulation.

2024-08-24 | GSE274191 | GEO

RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [ChIP-seq]

2024-08-24 | GSE274190 | GEO

RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [EM-seq]

2024-08-24 | GSE274196 | GEO

RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [smallRNA-seq]

2024-08-24 | GSE274192 | GEO

RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [Oxford Nanopore Technology Direct RNA Sequencing]

2024-08-24 | GSE274200 | GEO

RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [smallRNA-seq]

Project description:RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [smallRNA-seq]

| PRJNA1145379 | ENA

RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [ChIP-seq]

Project description:RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [ChIP-seq]

| PRJNA1145381 | ENA

RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [EM-seq]

Project description:RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [EM-seq]

| PRJNA1145382 | ENA

RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [Oxford Nanopore Technology Direct RNA Sequencing]

Project description:RNA quality control by CCR4 safeguards chromatin integrity and centromere function in Arabidopsis [Oxford Nanopore Technology Direct RNA Sequencing]

| PRJNA1145390 | ENA

CENPA as a reader of m6A-modified centromeric RNA to ensure centromere integrity in cancer cells [CHIP-seq]

Project description:Maintaining centromere integrity is crucial for genome stability and proper chromosome segregation during mitosis. CENPA, a conserved histone H3 variant, is localized at the centromeres and plays an essential role in preserving centromere integrity and function. However, the mechanisms that retain CENPA at centromeres remain an enigma. In this study, we identified CENPA as an m6A reader of centromeric RNA (cenRNA), which is essential for its centromeric localization and function in maintaining centromere stability. We discovered a higher level of m6A modification on cenRNA in cancerous cells compared to normal cells. CENPA preferentially binds the cenRNAs with m6A modification, which stabilizes its localization in centromeric regions during the S phase of the cell cycle. We then identified two residues in CENPA responsible for m6A recognition. Mutations in these CENPA residues or removal of m6A on cenRNAs leads to the loss of centromere-bound CENPA during the S phase, thereby resulting in abnormal chromosome separation during mitosis and increased genomic instability. Consequently, this impairment in centromere integrity hindered cancer cell proliferation and tumor growth. Our findings unveiled a novel m6A reading mechanism by CENPA that epigenetically governs centromere integrity in cancer cells, providing potentially new targets for cancer therapy.

2024-09-24 | GSE230879 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data