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NOTCH, ERK and SHH signaling respectively control the fate determination of cortical astrocytes, oligodendrocytes, and olfactory bulb interneurons

ABSTRACT: NOTCH, ERK and SHH signaling respectively control the fate determination of cortical astrocytes, oligodendrocytes, and olfactory bulb interneurons

PROVIDER: PRJNA1147184 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30218814_1.fastq.gz	Fastqsanger.gz
	SRR30218814_2.fastq.gz	Fastqsanger.gz
	SRR30218815_1.fastq.gz	Fastqsanger.gz
	SRR30218815_2.fastq.gz	Fastqsanger.gz
	SRR30218816_1.fastq.gz	Fastqsanger.gz

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NOTCH, ERK and SHH signaling respectively control the fate determination of cortical astrocytes, oligodendrocytes, and olfactory bulb interneurons

Project description:During cortical development, radial glial cells, known as neural stem cells, initially generate a large number of cortical glutamatergic pyramidal neurons through a process called neurogenesis. This is followed by the generation of a diversity of cortical astrocytes, oligodendrocytes, and olfactory bulb interneurons, known as gliogenesis. However, the molecular mechanisms underlying the switch from cortical neurogenesis to gliogenesis, and the subsequent fate determination of cortical astrocytes, oligodendrocytes, and olfactory bulb interneurons, remain unclear. Here, we report that extracellular signal-regulated kinase (ERK) signaling plays a fundamental role in promoting cortical gliogenesis and the generation of cortical glial progenitors. Additionally, SHH-SMO-GLI activator signaling has an auxiliary function to ERK during these processes. We further demonstrate that NOTCH signaling is absolutely required for the fate determination of astrocytes, while ERK signaling plays a prominent role in oligodendrocyte fate specification, and SHH signaling is crucial for the fate determination of olfactory bulb interneurons from cortical progenitors. We provide evidence suggesting that this mechanism is conserved in both mice and humans. Finally, we propose a unifying principle of mammalian cortical gliogenesis.

2024-08-23 | GSE274547 | GEO

NOTCH, ERK and SHH signaling respectively control the fate determination of cortical astrocytes, oligodendrocytes, and olfactory bulb interneurons [CUT&Tag]

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NOTCH, ERK and SHH signaling respectively control the fate determination of cortical astrocytes, oligodendrocytes, and olfactory bulb interneurons [CUT&Tag]

Project description:NOTCH, ERK and SHH signaling respectively control the fate determination of cortical astrocytes, oligodendrocytes, and olfactory bulb interneurons [CUT&Tag]

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Dlx1/2 Are Central and Essential Components in the Transcriptional Code for Generating Olfactory Bulb Interneurons

Project description:Generation of olfactory bulb (OB) interneurons requires neural stem/progenitor cell specification, proliferation, differentiation, and young interneuron migration and maturation. Here, we show that the homeobox transcription factors Dlx1/2 are central and essential components in the transcriptional code for generating OB interneurons. In Dlx1/2 constitutive null mutants, the differentiation of GSX2+ and ASCL1+ neural stem/progenitor cells in the dorsal lateral ganglionic eminence is blocked, resulting in a failure of OB interneuron generation. In Dlx1/2 conditional mutants (hGFAP-Cre; Dlx1/2F/- mice), GSX2+ and ASCL1+ neural stem/progenitor cells in the postnatal subventricular zone also fail to differentiate into OB interneurons. In contrast, overexpression of Dlx1&2 in embryonic mouse cortex led to ectopic production of OB-like interneurons that expressed Gad1, Sp8, Sp9, Arx, Pbx3, Etv1, Tshz1 and Prokr2. Pax6 mutants generate cortical ectopia with OB-like interneurons, but do not do so in compound Pax6; Dlx1/2 mutants. We propose that DLX1/2 promote OB interneuron development mainly through activating the expression of Sp8/9, which further promote Tshz1 and Prokr2 expression. Based on this study, in combination with earlier ones, we propose a transcriptional network for the process of OB interneuron development. This SuperSeries is composed of the SubSeries listed below.

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Olig2 Defines a Unique Subset of Neural Stem Cells That Produce Specific Olfactory Bulb Interneuron Subtypes in the Subventricular Zone of Postnatal and Adult Mice

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