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Methylorubrum extorquens PA1 breed:bacteria

ABSTRACT: Whole genome sequencing of Methylorubrum extorquens PA1 strain deleted fdh1, fdh2, fdh3, and fdh4, 16 weeks of continuous transfer in formate-containing medium - Raw sequence reads

PROVIDER: PRJNA1148342 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30718061_1.fastq.gz	Fastqsanger.gz
	SRR30718061_2.fastq.gz	Fastqsanger.gz

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Methylorubrum extorquens PA1 breed:bacteria

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Project description:Methylorubrum extorquens AM1 is engineered to produce itaconic acid by heterologous expression of cis-aconitic acid decarboxylase. Mutation was also performed on phaR in Methylorubrum extorquens AM1, which regulate poly-beta-hydroxybutyrate accumulation, in attempt to increase carbon flux toward itaconic acid production. However, in our case, itaconic acid production by phaR mutant strain was not higher than that of the wildtype. Transcriptomic analysis was utilized in order to examine the cause for this phenomenon. RNA-seq analysis revealed that phaR mutation in the itaconic acid-producing strain might result in a complex regulatory rewiring at the gene expression level, which could cause a reduced resource flux toward ITA production. Also, RNA profiling gave a hint at the broad regulatory role of PhaR.

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