Project description:To address whether, after depletion by Csf1r inhibition, microglia repopulating the CNS revert to a “younger” phenotype, we treated a group of 23 months old mice with the Csf1r inhibitor for 5 days and let microglia repopulate the central nervous system for 7 days. Young (3 months old) and old (23 months old) mice were included as controls. After the repopulation time, whole spinal cord samples and Fluorescence Activated Sorting (FACS)-sorted microglia cells from brain tissue were collected to perform bulk transcriptome (RNAseq) analysis. Analysis of whole spinal cord suggested that repopulating microglia tend to re-acquire its original aged phenotype. Gene expression in FACS-sorted microglia was then analyzed to better characterize its intrinsic phenotype after repopulation. In contrast to the whole spinal cord samples, old and old-treated replicates separated in Principal Component Analysis, suggesting that repopulating microglia displayed a different phenotype compared to old microglia.
Project description:To examine the effect of loss of dFOXO on the small RNA landscape of aging animals, we sequenced total small RNA collected from whole wildtype (wDah) and dFOXO-null (dfoxoΔ94) flies at young (5-6 days) and old (31 days) age.
Project description:Female ICR mice 6-8 weeks old and weighing 23-28 grams received a mild traumatic brain injury via weight drop model. 48 hours after trauma, the mice were sacrificed and RNA was extracted from lungs to examine what pulmonary gene expression changes may have been triggered by the brain injury using microarray analysis.
Project description:A total of 30 days of exposure to cigarette smoke (CS) or indoor air was given to 8- 23 week-old female C57BL/6 mice, then proteomics analyses were used to determine the 24 impact of CS on ovarian reserve.
