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ABSTRACT: Marchantia polymorpha RNAseq

PROVIDER: PRJNA1149659 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR30294801_1.fastq.gz	Fastqsanger.gz
	SRR30294801_2.fastq.gz	Fastqsanger.gz
	SRR30294802_1.fastq.gz	Fastqsanger.gz
	SRR30294802_2.fastq.gz	Fastqsanger.gz
	SRR30294803_1.fastq.gz	Fastqsanger.gz

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Project description:Marchantia polymorpha RNAseq

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Transcriptome changes triggered by the loss of function of gene Mppcs in genome-edited Marchantia polymorpha plants

Project description:In the present study, Marchantia polymorpha Mppcs loss of function mutants were generated through CRISPR/cas9 mediated genome-editing. To assess whether the knockout of MpPCS gene affects the transcription of M. polymorpha nuclear genes in unstressed condition, the Mppcs-2 knockout mutant and Cam2 wild-type transcriptomes were compared by RNA-Seq.

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Project description:RNA-seq of Marchantia polymorpha Mpb-gata1 mutants was performed in order to investigate their molecular signature of gene expression changes.

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Project description:To evaluate role of PRC2 on transcriptional regulation in bryophytes, we generated loss of function mutants of a Ez homolog in Marchantia polymorpha by CRISPR/Cas9 based mutagenesis. To avoid lethality of PRC2 knockout, we first mutate MpKNOX2 gene then additionally knocked MpEz1 out. Then we performed comparative transcriptome analyses using data obtained from Mpknox2 ez1 and Mpknox2.

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