Project description:Wild type Lec1 transgene induction by DEX: Establishing the network of seed gene expression and analysis of its biodiversity (wt dex induction)
Project description:gnp3_tri33-arabidoseed - preliminary experiment:repetability - WP3 : Biodiversity of seed traits : state of the art - The third experiment is a comparison of the transcriptome of two parents (Bay0,Sha) and two RILs (104, 140) in loop experimental design. Plants are grown in individual pots on a tray.Flowers were tagged on the flowering day using colored tape. Siliques were harvested 10 days after flowering, freezed in liquid nitrogene and stored at -80degreeC until RNA extraction. Keywords: genotype and ecotype comparison
Project description:Seed development, which depends on mother plant genetic background and environmental conditions, is a major component determining seed composition. Seed quality is a main agricultural concern, impacting both food and non-food applications, while also playing a central role in biodiversity conservation and environment protection. Climate change, characterized, among other stresses, by the emergence of extremely high temperatures, constitute a critical global threat to agriculture. Specialized metabolites (SMs) play crucial roles in the interactions of plants and seeds with their environments. Several SMs are known to be protective compounds involved in seed stress responses, thus impacting directly or indirectly their quality. In this study, we performed untargeted metabolomic (LC-MS/MS) and transcriptomic (RNA-Seq) analyses of Arabidopsis thaliana seeds harvested at six developmental stages (Globular, Transition, Torpedo, Bent cotyledon, Mature green and Dry seed), and developed under control and warm temperature conditions. Those data provide an original and valuable resource for future studies on the role of SMs and genes involved in seed warm thermic stress responses and for the study of their regulation and functions during seed development.
Project description:gnp3_tri33-arabidoseed - preliminary experiment:repetability - WP3 : Biodiversity of seed traits : state of the art - The third experiment is a comparison of the transcriptome of two parents (Bay0,Sha) and two RILs (104, 140) in loop experimental design. Plants are grown in individual pots on a tray.Flowers were tagged on the flowering day using colored tape. Siliques were harvested 10 days after flowering, freezed in liquid nitrogene and stored at -80degreeC until RNA extraction. Keywords: genotype and ecotype comparison 8 dye-swap - CATMA arrays
