Project description:Wild type Lec1 transgene induction by DEX: Establishing the network of seed gene expression and analysis of its biodiversity (wt dex induction)
Project description:Seed development, which depends on mother plant genetic background and environmental conditions, is a major component determining seed composition. Seed quality is a main agricultural concern, impacting both food and non-food applications, while also playing a central role in biodiversity conservation and environment protection. Climate change, characterized, among other stresses, by the emergence of extremely high temperatures, constitute a critical global threat to agriculture. Specialized metabolites (SMs) play crucial roles in the interactions of plants and seeds with their environments. Several SMs are known to be protective compounds involved in seed stress responses, thus impacting directly or indirectly their quality. In this study, we performed untargeted metabolomic (LC-MS/MS) and transcriptomic (RNA-Seq) analyses of Arabidopsis thaliana seeds harvested at six developmental stages (Globular, Transition, Torpedo, Bent cotyledon, Mature green and Dry seed), and developed under control and warm temperature conditions. Those data provide an original and valuable resource for future studies on the role of SMs and genes involved in seed warm thermic stress responses and for the study of their regulation and functions during seed development.
Project description:Transcriptional profiling in the shoot after SHR induction by DEX. We used Affymetrix ATH1 microarrays to identify new target genes of SHR and the effect of SHR on growth and development of the Arabidopsis shoot system by global transcriptome analysis.
Project description:wt dex induction - Identification of LEC1 target genes - Induction of 14 days old WT seedlings , different treatments ( EtOH, DEX induction) for 8 hours
