Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression in Phf6-deleted and Phf6-intact foetal mouse cortical neurons and foetal mouse cortex assessed by RNA-seq

ABSTRACT: Gene expression in Phf6-deleted and Phf6-intact foetal mouse cortical neurons and foetal mouse cortex assessed by RNA-seq

PROVIDER: PRJNA1152848 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30411900_1.fastq.gz	Fastqsanger.gz
	SRR30411900_2.fastq.gz	Fastqsanger.gz
	SRR30411901_1.fastq.gz	Fastqsanger.gz
	SRR30411901_2.fastq.gz	Fastqsanger.gz
	SRR30411902_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

Gene expression in Phf6-deleted and Phf6-intact foetal mouse cortical neurons and foetal mouse cortex assessed by RNA-seq

Project description:This study assessed the impact of germline Phf6-deletion on gene expression in the E15.5 mouse cortex and the impact of Nestin-cre mediated Phf6-deletion on gene expression in cultured E16.5 mouse cortical neurons

2024-09-23 | GSE275729 | GEO

RNA-seq profiling of HSC, MPPs and HPC-1s from Phf6-deleted and Phf6-intact mice

Project description:The study profiled the effect of Phf6 deletion on gene expression in hematopoietic stem cells (HSCs), multipotent progenitor cells (MPPs) and hematopoietic progenitor cells (HPC-1). Phf6lox/Y;Tie2-creTg/+ mice were prepared on a C57BL/6 background so that Phf6 deletion could be selectively mediated by Tie2-cre. Cell populations were sorted from bone marrow samples using standard surface markers (Lin–SCA1+cKIT+(LSK) CD150+ CD48– for HSCs, Lin–SCA1+cKIT+(LSK) CD150– CD48– for MPPs and Lin–SCA1+cKIT+(LSK) CD150- CD48+ for HPC-1 cells). Phf6 intact and Phf6-delected cells of all three types were profiled by paired-end RNA-seq using an Illumina NextSeq 500 sequencer. RNA-seq libraries were prepared from the HPC-1 samples used a standard Illumina TruSeq library protocol whereas the libraries for the HSC and MPP samples used a SMART-seq ultra low input kit for cDNA synthesis and amplification. Statistical analysis of the TruSeq and SMART-seq samples was undertaken separately.

2019-02-20 | GSE126316 | GEO

RNA-seq profiling of HSC, MPPs and HPC-1s from Phf6-deleted and Phf6-intact mice

Project description:RNA-seq profiling of HSC, MPPs and HPC-1s from Phf6-deleted and Phf6-intact mice

| PRJNA521533 | ENA

RNA sequencing of isogenic Phf6 wild type and Phf6 knockout mouse T-ALL cells

Project description:Loss-of-function mutations Phf6 occur frequently in both adult and pediatric T-cell Acute Lymphoblastic Leukemia (T-ALL). Although Phf6 is widely expressed, little is known about its proposed function as epigenetic regulator and tumor suppressor. To address the role of Phf6 in the leukemia development, here we analyze by RNAseq the gene expression profiles of isogenic Phf6 wild type and Phf6 knockout leukemia cells transformed by overexpression of an oncogenic mutant form of the NOTCH1 receptor.

2019-07-31 | GSE117165 | GEO

Transcriptomic changes in ER-HOXB8 mouse cell line on Phf6 knockout

Project description:PHF6 is a transcriptional regulator mutated in 3-5% of acute myeloid leukemia. To understand the effect of loss and clinical mutation of PHF6 on the transcriptome of myeloid cells we generated multiple wildtype and Phf6 knockout clones. We observed that loss of PHF6 upregulated genes related to stemness and downregulates genes related to myeloid differentiation.

2024-11-15 | GSE281625 | GEO

ATAC-Seq of foetal blood progenitors

Project description:Chromatin accessibility was assessed by ATAC-Seq in lymphoid-primed multipotent progenitors (LMPPs) from human foetal livers (FLs) and mouse wild-type FLs as well as FLs from mouse embryos that express the oncofusion Mll-AF4 in the definitive blood system. The aim of this study was to establish whether overall chromatin accessibility at key haematopoietic sites and loci that have been linked to leukaemia are differentially accessible in human vs mouse LMPPs and whether this is altered by the expression of the Mll-AF4 oncofusion.

2021-05-28 | GSE168438 | GEO

PHF6 OCCUPANCYIN HUMAN T-CELL ACUTE LYMPHOBLASTIC LEUKEMIA

Project description:Here we identify the genome-wide occupancy of PHF6 in T-ALL cells. Human T-ALL cells were cross-linked with formaldehyde for 20 min. DNA was enriched by chromatin immunoprecipitation (ChIP) and analyzed by Solexa sequencing. A sample of whole cell extract (WCE) was sequenced and used as the background to determine enrichment. ChIP was performed using an antibody against total PHF6 (Bethyl A301-451A). This represents the ChIP-seq portion of this dataset.

2014-04-01 | E-GEOD-45864 | biostudies-arrayexpress

RNA sequencing of isogenic Phf6 wild type and Phf6 knockout mouse T-ALL cells

Project description:RNA sequencing of isogenic Phf6 wild type and Phf6 knockout mouse T-ALL cells

| PRJNA481336 | ENA

Gene regulation of PHF6 in mouse developing cortex

Project description:we performed RNAseq between WT/KO and WT/C99F to understand the function of PHF6 in gene regulation

2018-10-30 | GSE119973 | GEO

Gene regulation of PHF6 in mouse developing cortex

Project description:Gene regulation of PHF6 in mouse developing cortex

| PRJNA490879 | ENA