Project description:Unbiased and FACS was performed on human intestinal organoid cells and coupled to single cell sorting by the SortSeq protocol (Muraro et al., 2016).
Project description:We focused on a rare cell population of human intestine, the M cells. Using human intestinal organoid as a model, the M cells could be differentiated, FACS-sorted from organoids and analyzed by scRNA-seq.
Project description:We focused on a rare cell population of human intestine, the BEST4+ cells. Using human intestinal organoid as a model, the BEST4+ cells could be differentiated, FACS-enriched and analyzed by scRNA-seq, together with the BEST4- cell lineages such as the enterocytes, goble cells and EECs.
Project description:We focused on a rare cell population of human intestine, the M cells. Using human intestinal organoid as a model, the M cells could be differentiated, FACS-enriched and analyzed by RNA-seq.
Project description:In this project, we aimed to examine the transcriptional changes that occur after irradiation of intestinal organoid-derived subcutaneous heterotopic tumors over a 7 day period post-radiation treatment. AKPT (villinCreER;Apcfl/fl;KrasG12D/+;Trp53fl/fl;TgfbrIfl/fl) intestinal organoids were cultured, then suspended in a 50:50 phosphate buffered-saline and Matrigel mixture and subcutaneously implanted into male C57BL/6 mice. Two weeks after implantation, mice were given either a single dose of 15 Gy radiation or 3 doses of 7 Gy radiation. Tumours were harvested 4 hours, 24 hours, 3 days, and 7 days after receiving the single dose of 15 Gy or the final dose of 7 Gy radiation, as well as from corresponding non-irradiated controls. RNA was extracted from the collected tumours and processed for RNA sequencing.