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ABSTRACT: Transcriptome sequencing of Helicoverpa armigera larvae following starvation

PROVIDER: PRJNA1153293 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR30427203_1.fastq.gz	Fastqsanger.gz
	SRR30427203_2.fastq.gz	Fastqsanger.gz
	SRR30427204_1.fastq.gz	Fastqsanger.gz
	SRR30427204_2.fastq.gz	Fastqsanger.gz
	SRR30427205_1.fastq.gz	Fastqsanger.gz

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Identification and expression profiling of Helicoverpa armigera microRNAs and their possible role in the regulation of digestive protease genes

Project description:Using Illumina platform, deep sequencing of 12 small RNA libraries was performed from Helicoverpa armigera larvae fed on artificial diet (AD) or recombinant Capsicum annuum PI-7 (rCanPI-7) incorporated diet at various time intervals (0.5, 2, 6, 12, 24, and 48h). Several candidate miRNAs (conserved and novel) were differentially expressed in rCanPI-7 fed larvae as compared to the larvae fed on AD. The investigation revealed potential roles of miRNAs in H. armigera protease gene regulation.

2017-09-15 | GSE61503 | GEO

Histone deacetylase 3: A potential target fight against Helicoverpa armigera

Project description:Insect development requires genes to be expressed in strict spatiotemporal order. The degree of histone acetylation regulates insect development, via histone acetyltransferases (HATs) and histone deacetylases (HDACs). Although HDAC3 is required for early embryonic development, its functions in Helicoverpa armigera remain unclear. We treated H. armigera with HDAC3 siRNA and RGFP966, a specific inhibitor, examining how HDAC3 loss-of-function affects growth and development. HDAC3 siRNA and RGFP966 treatment increased mortality at each growth-stage and altered metamorphosis, hampering pupation and causing abnormal wing development, reduced egg production, and reduced hatching rate. RNA-seq analysis identified 2,788 differentially expressed genes (≥ two-fold change; P ≤ 0.05) between siHDAC3- and siNC-treated larvae. Kr-h1, were differentially expressed in HDAC3 knockdown larvae. Pathway enrichment analysis revealed significant enrichment of genes involved in the Hippo, MAPK, and Wnt signaling pathways following HDAC3 knockdown. Histone H3K9 acetylation was increased in H. armigera after siHDAC3 treatment. In conclusion, HDAC3-knockdown dysregulated 20-hydroxyecdysone hormone-related and apoptosis-related genes in H. armigera, affecting many basic processes, including cell cycle regulation, metabolism, and signal transduction. The Result showed that HDAC3 gene can serve as a potential target for fighting against Helicoverpa armigera.

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Genome scale analysis of host-virus mRNA differential expression in Helicoverpa zea insect cells infected by H. armigera baculovirus from early till late infection

2013-03-01 | GSE44184 | GEO

Integrated transcriptomic and proteomic analyses revealed the participation of endogenous protease inhibitors in the regulation of protease gene expression in Helicoverpa armigera

Project description:Insects adapt to plant protease inhibitors (PIs) present in their diet by differentially regulating multiple digestive proteases. However, mechanisms regulating protease gene expression in insects are largely enigmatic. Ingestion of Capsicum annuum protease inhibitor-7 (CanPI-7) arrests growth and development of Helicoverpa armigera (Lepidoptera: noctuidae). Using de novo RNA sequencing and proteomic analysis, we examined the response of H. armigera larvae fed on recombinant C. annuum PI (CanPI) at different time intervals. Here, we present evidence supporting a dynamic transition in H. armigera protease expression upon CanPI feeding with general down-regulation of protease genes at early time points (0.5 to 6 h) and significant up-regulation of specific trypsin, chymotrypsin and aminopeptidase genes at later time points (12 to 48 h).

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Transcriptional analysis of physiological pathways in a generalist herbivore: responses to different host plants and plant structures by the cotton bollworm (CBW) Helicoverpa armigera

Project description:This SuperSeries is composed of the following subset Series: GSE34344: Transcriptional analysis of physiological pathways in a generalist herbivore: responses to different host plants and plant structures by the cotton bollworm (CBW) Helicoverpa armigera [CottonStructures] GSE34346: Transcriptional analysis of physiological pathways in a generalist herbivore: responses to different host plants and plant structures by the cotton bollworm (CBW) Helicoverpa armigera [DifferentHost] Refer to individual Series

2011-12-12 | E-GEOD-34349 | biostudies-arrayexpress

Helicoverpa armigera

Project description:Transcriptome Analysis of Helicoverpa armigera Larvae

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Genome-wide characterisation of DNA methylation in an invasive Lepidopteran pest, the cotton bollworm Helicoverpa armigera

Project description:Whole genome sequence data from bisulfite-treated genomic DNA extracted from adult Helicoverpa armigera moths.

2018-01-19 | E-MTAB-4779 | biostudies-arrayexpress

Project description:Transcriptome sequencing of the midgut from Helicoverpa armigera larvae

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Project description:Pesticide treatment of Helicoverpa armigera larvae

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Genome scale analysis of host-virus mRNA differential expression in Helicoverpa zea insect cells infected by H. armigera baculovirus from early till late infection

Project description:A custom microarray, based on deep transcriptome sequencing ((GEO accession number: GSE34418), was used to simultaneously investigate expression of over 24,000 Helicoverpa zea insect transcripts and 134 H. armigera nucleopolyhedrovirus (HearNPV) genes throughout the infection process at 0, 12, 24 and 48 hours post infection A cutom 8x60,000 SurePrint Agilent expression slide (Agilent, Santa Clara, CA) was used to analyzed eight samples, including biological replicates of HaSNPV-infected cultures at 0, 12, 24 and 48 hpi.The microarray probes included probes for 27,400 H. zea sequences that were validated previously (Nguyen et al., 2012. PLOS ONE, 7(5), e36324) and all 134 H. armigera single-capsid nucleopolyhedrovirus (HearNPV) genes (Accession: NC_002654).

2013-03-01 | E-GEOD-44184 | biostudies-arrayexpress

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