Proteomics

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Integrated transcriptomic and proteomic analyses revealed the participation of endogenous protease inhibitors in the regulation of protease gene expression in Helicoverpa armigera

ABSTRACT: Insects adapt to plant protease inhibitors (PIs) present in their diet by differentially regulating multiple digestive proteases. However, mechanisms regulating protease gene expression in insects are largely enigmatic. Ingestion of Capsicum annuum protease inhibitor-7 (CanPI-7) arrests growth and development of Helicoverpa armigera (Lepidoptera: noctuidae). Using de novo RNA sequencing and proteomic analysis, we examined the response of H. armigera larvae fed on recombinant C. annuum PI (CanPI) at different time intervals. Here, we present evidence supporting a dynamic transition in H. armigera protease expression upon CanPI feeding with general down-regulation of protease genes at early time points (0.5 to 6 h) and significant up-regulation of specific trypsin, chymotrypsin and aminopeptidase genes at later time points (12 to 48 h).

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Helicoverpa Armigera (cotton Bollworm)

TISSUE(S): Whole Body, Blood

SUBMITTER: Ashok Giri  

LAB HEAD: Ashok P. Giri

PROVIDER: PXD008376 | Pride | 2018-04-25

REPOSITORIES: Pride

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EC-2_IDA_New.wiff Wiff
EC-2_IDA_New.wiff.scan Wiff
EC_1_IDA.wiff Wiff
EC_1_IDA.wiff.scan Wiff
EC_1_IDA_New.wiff Wiff
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Integrated Transcriptomic and Proteomic Analyses Suggest the Participation of Endogenous Protease Inhibitors in the Regulation of Protease Gene Expression in <i>Helicoverpa armigera</i>.

Lomate Purushottam R PR   Dewangan Veena V   Mahajan Neha S NS   Kumar Yashwant Y   Kulkarni Abhijeet A   Wang Li L   Saxena Smita S   Gupta Vidya S VS   Giri Ashok P AP  

Molecular & cellular proteomics : MCP 20180416 7

Insects adapt to plant protease inhibitors (PIs) present in their diet by differentially regulating multiple digestive proteases. However, mechanisms regulating protease gene expression in insects are largely enigmatic. Ingestion of multi-domain recombinant <i>Capsicum annuum</i> protease inhibitor-7 (CanPI-7) arrests growth and development of <i>Helicoverpa armigera</i> (Lepidoptera: Noctuidae). Using <i>de novo</i> RNA sequencing and proteomic analysis, we examined the response of <i>H. armige  ...[more]

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