Project description:Streptococcus gallolyticus subsp. gallolyticus is a commensal of the human gastrointestinal tract and a pathogen of infective endocarditis and other biofilm-associated infections with exposed collagen. Therefore, this study focuses on the characterization of the biofilm formation and collagen adhesion of S. gallolyticus subsp. gallolyticus under different conditions. It has been observed that lysozyme triggers biofilm formation divergently in the analyzed S. gallolyticus subsp. gallolyticus strains. The transcriptome analysis was performed for two strains which form more biofilm in the presence of lysozyme. Lysozyme leads to higher expression of genes of transcription and translation, of the dlt operon (cell wall modification), of hydrogen peroxide resistance proteins and of two immunity proteins which could be involved in biofilm formation. Furthermore, the adhesion ability of 73 different S. gallolyticus subsp. gallolyticus strains to collagen type I and IV was analyzed. High adhesion ability was observed for the strain UCN 34, whereas the strain DSM 16831 adhered only marginally to collagen. The full genome microarray analysis revealed strain-dependent gene expression due to adhesion. The expression of genes of a transposon and a phage region in strain DSM 16831 were increased, which corresponds to lateral gene transfer. Adherence to collagen leads to a change in the expression of genes of nutrients uptake in the strain UCN 34.

2017-05-17 | GSE98953 | GEO

Limosilactobacillus reuteri strain:BG-R33 (DSM 33633)

Project description:Limosilactobacillus reuteri strain:BG-R33 (DSM 33633) Genome sequencing

| PRJNA1138931 | ENA

Limosilactobacillus reuteri subsp. kinnaridis strain:BG-R46

Project description:Limosilactobacillus reuteri subsp. kinnaridis strain:BG-R46 Genome sequencing

| PRJNA1153400 | ENA

RNA-seq analysis on the liver of darkness rats treated with Limosilactobacillus reuteri

Project description:We have previously demonstrated that the arrhythmic expressions of circadian clock genes due to constant darkness induce glycometabolic and reproductive hallmarks of polycystic ovary syndrome (PCOS) in rats. Limosilactobacillus reuteri (L.reuteri) is a promising dietary intervention for host dysmetabolism, while its potential effect on circadian dysrhythmia-induced PCOS remains elusive. Here, we evaluated the amelioration of L.reuteri regimen on constant darkness-induced PCOS-like rats through detecting hepatic gene expression profiles by RNA-seq.

2023-07-11 | GSE169501 | GEO

Gene expression comparison of L. reuteri DSM 17938 and pocR mutant

Project description:Comparison of gene expression between L. reuteri DSM 17938 and L. reuteri DSM 17938::pocR mutant grown in semi-defined medium after 24h of growth at 37C in anaerobic condition. PocR is an AraC-like transcriptional regulator, and changes in gene expression between mutant and wild-type strains would indicate genes involved in the PocR regulon. Includes 3 biological replicates and dye-swaps for DSM 17938 versus pocR mutant. One sample includes total RNA isolated from wildtype DSM 17938 labeled with either cy3 or cy5, and total RNA isolated from the pocR mutant labeled with the opposite dye. Samples 1, 2, and 3 represent biological replicates. Samples 4, 5, and 6 represent dye-swaps of the same biological replicates.

2014-01-24 | E-GEOD-54324 | biostudies-arrayexpress

Limosilactobacillus reuteri strain:ZLR003

Project description:Limosilactobacillus reuteri strain:ZLR003 Assembly

| PRJNA290298 | ENA

Limosilactobacillus reuteri

Project description:Limosilactobacillus reuteri Genome sequencing

| PRJNA994435 | ENA

Limosilactobacillus reuteri

Project description:Limosilactobacillus reuteri Genome sequencing

| PRJNA1067331 | ENA