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ABSTRACT: RNA-seq of cheese starter cultures

PROVIDER: PRJNA1157897 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR30648944.fastq.gz	Fastqsanger.gz
	SRR30648945.fastq.gz	Fastqsanger.gz
	SRR30648946.fastq.gz	Fastqsanger.gz
	SRR30648947.fastq.gz	Fastqsanger.gz
	SRR30648948.fastq.gz	Fastqsanger.gz

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Project description:Propionibacterium freudenreichii, a dairy starter, reaches a population close to 10^9 propionibacteria per gram of Swiss-type cheese at the time of consumption. Also consumed as a probiotic, it revealed strain-dependent anti-inflammatory properties mediated by proteins inducing IL-10 in leukocytes. Here, strains with varied anti-inflammatory potentials were compared in terms of transcriptome profiles.

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Project description:Propionibacterium freudenreichii is an important starter culture used in the manufacture of Swiss-type cheeses. We have generated the complete genome sequence of a Propionibacterium freudenreichii ssp. shermanii strain JS at the Institute of Biotechnology, University of Helsinki, by using a combination of pyrosequencing with GS FLX and GS FLX Titanium series reagents (Roche) and SOLiD 4 (Life Technologies), ABI 3130xl Genetic Analyzer (Life Technologies), and PacBio RS II (Pacific Biosciences) instruments. Initial genome annotation was carried out using RAST, and additional functional annotation information for each CDS was obtained from BLANNOTATOR, CDD, and KAAS. Accession number for genome sequence is PRJEB12148. This submission is for the transcriptome analysis of Propionibakcterium freudenreichii in cheese ripening under warm and cold conditions. The RNA reads were mapped to the reference genome PRJEB12148.

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