Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1159909 | ENA |
REPOSITORIES: ENA
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Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature.
2013-11-01 | GSE42557 | GEO
Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature.
2013-11-01 | GSE42556 | GEO
Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature.
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Project description:DNA oligonucleotide microarrays were designed with 307 probes for 96 internal transcribed spacer (ITS1, located between 18S and 26S rRNA genes) sequences of known species and strains from the genus Pseudo-nitzschia (Bacillariophyceae). In addition, microarrays also carried 1893 probes targeting ITS1 aequences of marine Crenarchaeota and Alphaproteobacteria of SAR11 clade. In order to assign microarray profiles to Pseudo-nitzschia ribotypes and species and to 'train' the data analysis system, we grew cultures of Pseudo-nitzschia in the laboratory with identities confirmed through rDNA sequence analysis. In total, 9 cultures and 35 environmental water samples were hybridized to microarrays, in some cases, in duplicate or triplicate. Analysis of microarray data allowed us to identify and map Pseudo-nitzschia spp. in the coastal waters along Washington and Oregon coast of the Eastern Pacific Ocean, and to observe seasonal changes in diatom community composition.
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Project description:Characterizing a common cellular stress response (CSR) to high water temperature across species and populations is necessary for identifying the capacity of Pacific salmon (Oncorhynchus spp.) to persist in current and future climate warming scenarios, especially for populations at the southern periphery of their species' distributions. In this study, populations of wild adult pink (O. gorbuscha) and sockeye (O. nerka) salmon from the Fraser River, British Columbia, Canada, were experimentally treated to an ecologically relevant 'cool' or 'warm' water temperature to uncover common transcriptomic responses to elevated water temperature. Ninety-eight samples from three separate temperature exposure studies were analyzed on ninety-eight microarrays, using a common reference design, with multiple biological replicates for each temperature condition for each year of the experiment.
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