Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Environmental alkalization suppresses deployment of virulence strategies in Pseudomonas syringae pv. tomato DC3000

ABSTRACT: Environmental alkalization suppresses deployment of virulence strategies in Pseudomonas syringae pv. tomato DC3000

PROVIDER: PRJNA1162728 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR30718900_1.fastq.gz	Fastqsanger.gz
	SRR30718900_2.fastq.gz	Fastqsanger.gz
	SRR30718901_1.fastq.gz	Fastqsanger.gz
	SRR30718901_2.fastq.gz	Fastqsanger.gz
	SRR30718902_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Environmental alkalization suppresses deployment of virulence strategies in Pseudomonas syringae pv. tomato DC3000

Project description:Plant pathogenic bacteria encounter a drastic increase in apoplastic pH during the early stages of plant immunity. The effects of alkalization on pathogen-host interactions have not been comprehensively characterized. Here we used a global transcriptomic approach to assess the impact of environmental alkalization on Pseudomonas syringae pv. tomato DC3000 in vitro. In addition to the Type 3 Secretion System (T3SS), we found expression of genes encoding other virulence factors such as iron uptake, and coronatine biosynthesis to be strongly affected by environmental alkalization. We also found activity of extracytoplasmic function sigma factor, AlgU, was induced at pH 5.5 and suppressed at pH 7.8, which are pH levels that this pathogen would likely experience before and during pattern triggered immunity, respectively. This pH-dependent control requires the presence of periplasmic proteases, AlgW and MucP, that function as part of the environmental sensing system that activates AlgU in specific conditions. This is the first example of pH-dependency of AlgU activity, suggesting a regulatory pathway model where pH affects the proteolysis-dependent activation of AlgU. These results contribute to deeper understanding of the role apoplastic pH has on host-pathogen interactions.

2024-10-03 | GSE277531 | GEO

Pseudomonas syringae pv. tomato DC3000 exploits light signals to optimize virulence and colonization of leaves

Project description:To explore the effect of light perception on Pseudomonas syringae pv. tomato DC3000 at a global level, we carried out microarray hybridization experiments. We hybridize custom-designed microarrays (Agilent Technologies) with probes isolated from PsPto after a 10 min treatment with either 20 μE/m2s blue light, 20 μE/m2s red light, 70 μE/m2s white light, or cells kept in the darkness.

2018-10-01 | GSE101783 | GEO

RNA-seq analysis of Pseudomonas syringae pv. tomato DC3000

Project description:This experiment analyses the expression data of the wild type P. syringae pv. tomato DC3000 grown in the absence and in the presence of phloretin and naringenin.

2018-12-30 | E-MTAB-6426 | biostudies-arrayexpress

Tomato prf3 and tft3 mutants response to Pseudomonas syringae pv. tomato (Pst) DC3000

Project description:Comparative transcriptomics between prf3, Prf-SBP-FLAG complemented lines as controls and tft3 2-2 line (CRISPR/Cas9 tomato mutant line in Rio Grande-prf3 Prf-SBP-FLAG complemented background), treated with either buffer (as control) or P. syringae DC3000 6 hours post infiltration.

2023-03-28 | GSE167378 | GEO

RNA-seq analysis of Pseudomonas syringae pv. tomato DC3000

Project description:This experiment analyses the expresssion data of the wild type P. syringae pv. tomato DC3000 compared with its fleQ mutant grown under two different conditions: liquid culture in minimal medium and swarming plates.

2015-12-31 | E-MTAB-3779 | biostudies-arrayexpress

Transcription profiling of Nicotinan benthamiana in response to Pectobacterium carotovorum WPP14 and Pseudomonas syringae pv. tomato DC3000

Project description:Transcription profiling of Nicotinan benthamiana in response to Pectobacterium carotovorum WPP14 and Pseudomonas syringae pv. tomato DC3000

2010-07-24 | E-TABM-752 | biostudies-arrayexpress

Transcriptional dynamics driving MAMP-triggered immunity and pathogen effector-mediated immunosuppression in Arabidopsis leaves following infection with Pseudomonas syringae pv. tomato DC3000.

Project description:High-resolution temporal transcriptomic analysis of Arabidopsis thaliana leaves during infection by Pseudomonas syringae DC3000 and DC3000hrpA-thaliana

2015-06-02 | GSE56094 | GEO

Pseudomonas syringae pv. tomato str. DC3000

Project description:Pseudomonas syringae DC3000 transcriptome - 2mem_Ps_Bt_12.5hr_BR4

| PRJNA439589 | ENA

Pseudomonas syringae pv. tomato str. DC3000

Project description:Pseudomonas syringae DC3000 transcriptome - 2mem_Ps_Bt_30hr_BR1

| PRJNA439594 | ENA

Pseudomonas syringae pv. tomato str. DC3000

Project description:Pseudomonas syringae DC3000 transcriptome - 2mem_Ps_Bt_35hr_BR2

| PRJNA439599 | ENA