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RNA-sequencing of inguinal white adipose tissue (iWAT) from kynurenine 3-monooxygenase (Kmo) knockout mice and wild-type littermates on chow and high-fat diets.

ABSTRACT: RNA-sequencing of inguinal white adipose tissue (iWAT) from kynurenine 3-monooxygenase (Kmo) knockout mice and wild-type littermates on chow and high-fat diets.

PROVIDER: PRJNA1163367 | ENA |

REPOSITORIES: ENA

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RNA-sequencing of inguinal white adipose tissue (iWAT) from kynurenine 3-monooxygenase (Kmo) knockout mice and wild-type littermates on chow and high-fat diets.

Project description:To investigate if chronically elevated circulating kynurenic acid (KYNA) and reduced quinolinic acid (QUIN) levels affect whole-body energy metabolism, we used a knockout-first gene targeting strategy to eliminate Kmo gene expression in mice. We conducted a series of metabolic assessments on male and female mice fed either a chow or high-fat diet (HFD) and performed RNA-sequencing on the inguinal white adipose tissue (iWAT) from male Kmo-defficient mice and their wild-type littermate controls on chow and high-fat diets.

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Kynurenine 3-monooxygenase upregulates pluripotent genes through β-catenin and promotes triple-negative breast cancer progression

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Project description:Under normal physiological conditions, the kynurenine pathway (KP) plays a critical role in generating cellular energy and catabolizing tryptophan. Under inflammatory conditions, however, there is an upregulation of the KP enzymes, particularly kynurenine 3-monooxygenase (KMO). KMO has garnered much attention due to its production of toxic metabolites that have been implicated in many diseases and disorders. With many of these illnesses having an inadequate or modest treatment, there exists a need to develop KMO inhibitors that reduce the production of these toxic metabolites. Though prior efforts to find an appropriate KMO inhibitor were unpromising, the development of a KMO crystal structure has provided the opportunity for a rational structure-based design in the development of inhibitors. Therefore, the purpose of this review is to describe the kynurenine pathway, the kynurenine 3-monooxygenase enzyme, and KMO inhibitors and their potential candidacy for clinical use.

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2024-03-31 | PXD030516 | JPOST Repository

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