Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Escherichia phage Lambda strain:DCP3 | isolate:DCP3

ABSTRACT: Escherichia phage Lambda strain:DCP3 | isolate:DCP3 Genome sequencing

PROVIDER: PRJNA1164418 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

The probiotic Escherichia coli strain Nissle 1917 combats the lambdoid bacteriophages stx and λ

Project description:The Escherichia coli strain Nissle 1917 (EcN) is used as a probiotic for the treatment of certain gastrointestinal diseases in several European and non-European countries. In vitro studies showed EcN to efficiently inhibit the production of Shiga toxin (Stx) by Stx producing E. coli (STEC) such as Enterohemorrhagic E. coli (EHEC). The occurrence of the latest EHEC serotype (O104:H4) responsible for the great outbreak in 2011 in Germany was due to the infection of an enteroaggregative E. coli by a Stx 2-encoding lambdoid phage turning this E. coli into a lysogenic and subsequently into a Stx producing strain. Since EHEC infected persons are not recommended to be treated with antibiotics, EcN might be an alternative medication. However, because a harmless E. coli strain might be converted into a Stx-producer after becoming host to a stx encoding prophage, we tested EcN for stx-phage genome integration. Our experiments revealed the resistance of EcN towards not only stx-phages but also against the lambda phage. This resistance was not based on the lack of or by mutated phage receptors. Rather the expression of certain genes (superinfection exclusion B (sieB) and a phage repressor (pr) gene) of a defective prophage of EcN was involved in the complete resistance of EcN to infection by the stx- and lambda phage. Obviously, EcN cannot be turned into a Stx producer. Furthermore, we observed EcN to inactivate phages and thereby to protect E. coli K-12 strains against infection by stx- as well as lambda-phages. Inactivation of lambda-phages was due to binding of lambda-phages to LamB of EcN whereas inactivation of stx-phages was caused by a thermostable protein of EcN. These properties together with its ability to inhibit Stx production make EcN a good candidate for the prevention of illness caused by EHEC and probably for the treatment of already infected people.

2018-06-14 | GSE109932 | GEO

Cao2010 - Epigenetic state of lysogeny in phage lambda

Project description:References: 1. Xiaomei Zhu, Lan Yin, Leroy Hood, David Galas and Ping Ao, Efficiency, Robustness and Stochasticity of Gene Regulatory networks in Systems biology: Lambda switch as a working example, 2006. 2. Adam Arkin, John Ross and Harley H. McAdams, Stochastic kinetic analysis of developmental pathway bifurcation in phage lambda-infected Escherichia coli cells, 1998, Genetics, 149: 1633-1648. 3. GenBank sequence: NC_001416 is the whole genome sequence of phage lambda.

2005-01-01 | MODEL1308080003 | BioModels

RNA-seq of Escherichia coli K-12 MG1655 and Stx2 phage lysogens

Project description:Here, we investigated the impact of Stx2 phage carriage on Escherichia coli (E. coli) K-12 MG1655 host gene expression. Using quantitative RNA-seq analysis, we compared the transcriptome of naïve MG1655 and the lysogens carrying the Stx2 phage of the 2011 E. coli O104:H4 outbreak strain or of the E. coli O157:H7 strain PA8, which share high degree of sequence similarity.

2019-06-17 | GSE126710 | GEO

Response of Escherichia coli after Phage T4 Ghost Adsorption

Project description:After the attachment of the lytic phage T4 to Escherichia coli cells, 1% E. coli cells showed an approximately 40-fold increase in mutant frequency. They were designated as mutator A global transcriptome analysis using microarrays was conducted to determine the difference between parental strain and mutators, and the host responce after adsorption of the phage and the ghost.

2018-11-29 | E-MTAB-5636 | biostudies-arrayexpress

Escherichia phage vB_EcoM-ZQ3 isolate:Escherichia phage

Project description:Escherichia phage vB_EcoM-ZQ3 isolate:Escherichia phage Genome sequencing

| PRJNA699937 | ENA

Escherichia phage CLiu-2021a isolate:Escherichia phage

Project description:Escherichia phage CLiu-2021a isolate:Escherichia phage Genome sequencing

| PRJNA698446 | ENA

Escherichia phage vB_EcoP-ZQ2 isolate:Escherichia phage

Project description:Escherichia phage vB_EcoP-ZQ2 isolate:Escherichia phage Genome sequencing

| PRJNA699927 | ENA

Lambdavirus lambda

Project description:Speciation phage lambda