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RNA sequencing data of dural macrophages isolated from brains of Cx3cr1-GFP mice at different ages.

ABSTRACT: RNA sequencing data of dural macrophages isolated from brains of Cx3cr1-GFP mice at different ages.

PROVIDER: PRJNA1165151 | ENA |

REPOSITORIES: ENA

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RNA sequencing data of dural macrophages isolated from brains of Cx3cr1-GFP mice at different ages.

Project description:While macrophages in the meningeal compartments of the central nervous system (CNS) has been comprehensively characterized under steady state, studying their contribution to physiological and pathological processes has been severely hampered by the lack of specific targeting tools in vivo. Recent findings have shown that the dural sinus and its adjacent lymphatic vessels act as a neuroimmune interface. Notably, the cellular and functional heterogeneity of extrasinusoidal dural macrophages outside this immune hub is currently unclear. Therefore, we comprehensively characterized these cells using single-cell transcriptomics, fate mapping, confocal imaging, clonal analysis and transgenic mouse lines. Extrasinusoidal dural macrophages were clearly distinct from leptomeningeal and CNS parenchymal macrophages in terms of their origin, expansion kinetics and transcriptional profiles. Lastly, functional studies demonstrated that during autoimmune neuroinflammation, extrasinusoidal dural macrophages perform efferocytosis of granulocytes. Our results highlight a previously unappreciated myeloid cell diversity and provide insights into the brain’s innate immune system.

2024-12-20 | GSE277981 | GEO

Gene Expression in Cx3cr1-GFP+ Cells in PS2APP Alzheimer's Disease mice

Project description:This project defines the microglial gene expression profile in a transgenic mouse model of Alzheimer's, compared to non-transgenic age-matched controls, at a time when amyloid pathology and microgliosis are rampant. Microglia were sorted live from one hemisphere of cerebral cortex, using GFP expressed from Cx3cr1 locus (mice have one intact copy of Cx3cr1). RNA was isolated from sorted microglia using RNeasy mini. Two groups, PS2APP and non-transgenic, with 9 mice/group, aged 14-15 months. The "SAMPLE_ID" sample characteristic is a sample identifier internal to Genentech. The ID of this project in Genentech's ExpressionPlot database is PRJ0006715

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RNA sequencing data of microglia, meningeal macrophages (mMΦ) and perivascular macrophages (pvMΦ) isolated from brains of Cx3cr1-GFP mice at different age.

Project description:RNA sequencing data of microglia, meningeal macrophages (mMΦ) and perivascular macrophages (pvMΦ) isolated from brains of Cx3cr1-GFP mice at different age.

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The dural innate immune barrier protects the CNS from systemic virus invasion [CD45_10X]

Project description:To investigate cellular landscape of dural immune cells, dural immune cells from 30 P28 male mice and 30 P7 male mice were FACS sorted and single-cell RNA seqs were performed

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An abundant tissue macrophage population in the adult murine heart

Project description:GFP+CD45+CD11b+ cells were isolated from from heterozygous adult Cx3cr1-GFP/+ mouse hearts, spleens and brains by fluorescence activated cell sorting to prepare RNA for gene array analysis.<br>Three independently isolated populations GFP+ (CD45+CD11b+) cells for each tissue were used as replicates.

2012-04-25 | E-MEXP-3347 | biostudies-arrayexpress

Gene expression profile at sorted microglia and border-associated macrophage with Ms4a3-Cre: R26-TdTomato: Cx3cr1-gfp bone marrow transplantation

Project description:After irradiation and bone-marrow transplantation, most of microglia and boarder-associated macrophages in the central nervous system is replaced by engranft cells. Taking advantages of Ms4a3-Cre: R26-TdTomato: Cx3cr1-gfp mice, we discriminated cells from monocyte precursors and hematopoietic stem cells with colors at 32 weeks following irradiation and bone-marrow transplantation. We sorted HSC-derived, monocyte-derived, and endogenouse microglia and leptomeningeal macrophages, and analyzed gene expression level between different cell populations using a bulk RNAseq method.

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Gene expression profile at single cell level of YS-derived cells and monocyte-derived cells from brain parenchyma and leptomeninges of Ms4a3-Cre: R26-TdTomato: Cx3cr1-gfp mice

Project description:Ms4a3-Cre: R26-TdTomato: Cx3cr1-gfp mice allow discrimination between YS-derivd and monocyte-derived macrophages in the brain parenchyma and leptomeninges by color. We used single cell RNAseq for RNA profiling to compare YS-derived micrglia, monocyte-derived microglia, YS-derived leptomeningeal macrophages, and monocyte-derived leptomeningeal macrophages.

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RNA-seq in central brains from Harpegnathos castes

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Different roles of dural macrophages subpopulations to systemic viral infection

Project description:To investigate different responses of dural macrophage subsets to systemic viral infection, we collected the dura mater from mice 12 h after systemic LCMV infection and macrophages were sorted

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Cx3cr1-deficient microglia exhibit a premature aging transcriptome

Project description:CX3CR1, one of the highest expressed genes in microglia in mice and humans, is implicated in numerous microglial functions. However, the molecular mechanisms underlying Cx3cr1 signaling are not well understood. Here, we analyzed transcriptomes of Cx3cr1-deficient microglia under varying conditions by RNA-Seq. In 2 mos mice, Cx3cr1 deletion resulted in the downregulation of a subset of immune-related genes, without substantial epigenetic changes in markers of active chromatin. Surprisingly, Cx3cr1-deficient microglia from young mice exhibited a transcriptome consistent with that of aged Cx3cr1-sufficient animals, suggesting a premature aging transcriptomic signature. Immunohistochemical analysis of microglia in young and aged mice revealed that loss of Cx3cr1 modulates microglial morphology in a compatible fashion. Our results suggest that CX3CR1 may regulate microglial function in part by modulating the expression levels of a subset of inflammatory genes during chronological aging, making Cx3cr1-deficient mice useful for studying aged microglia.

2019-10-01 | GSE130960 | GEO

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