Project description:In the present work, Abortiporus biennis, a white-rot fungus, was studied in regard to its lignocellulolytic enzymatic potential. Secretomics analyses, combined with biochemical methods, were employed to study the enzymatic machinery of the strain, after growth in corn stover cultures and xylose-based defined media. The results revealed the presence of all the necessary enzymatic activities for complete breakdown of the lignocellulosic substrate, while the prominent role of oxidative enzymes in the lignocellulolytic strategy of the strain became evident. Two novel laccases, AbiLac1 and AbiLac2, were isolated from the culture supernatant of this fungus.
Project description:Total RNA from rumen epithelial tissues of cows fed alfalfa hay (AL),Rice straw (RS) or Corn stover (CS)diet were sequenced using Illumina Hiseq 2000 system. For comparative analysis, differentially expressed genes were identified with edgeR.
Project description:Total RNA from duodenum, jejunum, liver and mammary gland tissues of cows fed alfalfa hay (AL),Rice straw (RS) or Corn stover (CS) diet were sequenced using Illumina HiSeq 2000 system. For comparative analysis, differentially expressed genes were identified with edgeR and SAS software.
Project description:The fermentation of Zymomonas mobilis 2032 was conducted in 6% and 9% glucan-loading AFEX-pretreated corn stover hydrolysates, as well as in 7% glucan-loading AFEX-pretreated switchgrass hydrolysate, and RNA samples were collected at both glucose and xylose growth stages. Here we present a comprehensive RNA-Seq data set at both growth stages.
Project description:Catheter ablation is an effective treatment to prevent recurrence of Atrial fibrillation (AF) and can be used to maintain sinus rhythm and improve symptoms of AF, but to some extent it can cause a range of adverse effects associated with catheter ablation. Pulsed electric field is a newer treatment modality to replace catheter ablation for atrial fibrillation due to its fewer side effects. Different from radiofrequency ablation, which destroys diseased myocardial tissue by thermal energy, pulsed electric field ablation achieves the purpose of atrial fibrillation ablation by inducing damage to diseased myocardial cells through irreversible electroporation. However, some experimental parameters and mechanism of pulsed electric fields remain unclear.
Project description:The biochemical and molecular basis of bee pollen under drying stress is not clear. In this study, metabonomics and proteomics were used to study the biochemical regulation and phenolic compound changes in the rape bee pollen of electrohydrodynamic drying. The results show that the direct contribution rate of ionic wind and electric field to drying characteristic parameters was 68.4% and 31.6% respectively. Electric field has a greater effect on the quality parameters. Specifically, compared with ionic wind, ribosome, citric acid cycle and proteasome pathway play a more positive role in regulating the abiotic and biological biochemical responses of electric field dehydration. The protein-phenolic interaction of rape bee pollen during EHD drying led to the change of free phenolic acid content and the differential accumulation of acidmetabolic characteristics such as L-phenylalanine, L-aspartic acid and L-pyroglutamic acid. In addition, TEM observation showed that EHD drying also destroyed the integrity of cell wall structure and tapetum. The results revealed the quality formation mechanism and biochemical regulation strategy of rape bee pollen during processing.
