Similar Datasets
Project description:Comparison of the binding of GOLDEN2-LIKE (GLK) transcription factors in tomato, tobacco, Arabidopsis, maize and rice, show that genome cis-variation caused wide-spread TF binding divergence, and most of the TF binding sites are genetically redundant.
2022-12-08 | GSE220115 | GEO
Project description:Hrip1 was isolated from Alternaria tenuissima and was found to induce systematic acquired resistance in tobacco. In rice plants, it was found to confer immunity against rice blast fungi. To understand the genomic signature of Hrip1-induced immunity, rice seedlings were treated with 30 nm Hrip1 protein and triplicate leaf samples were subjected to sequencing on the BIGSEQ500 platform. Two groups of rice seedlings were grown to the three-leaf stage. The experiment group was treated with 30nm Hrip1 protein while the control plants were treated with Tris-HCl buffer. Triplicate samples from both setups were taken at 6, 12, 24, and 48 hr after treatment. Total RNA was extracted and reverse transcribed in first strand cDNA as input material.
2022-08-21 | GSE211516 | GEO
Project description:Cigarette smoking is the leading cause of lung cancer worldwide. Carcinogens in smoke produced during the combustion of cigarette tobacco are responsible for airway epithelial changes underlying lung carcinogenesis. Reduction of harmful constituents by heating rather than combusting tobacco would be a sound strategy to reduce the risk for lung cancer. In this study we characterized the functional and molecular changes during long-term treatment of human bronchial epithelial cells with total particulate matter (TPM) from a new candidate modified risk tobacco product (cMRTP), the tobacco heated system 2.2 (THS2.2) in comparison with TPM from combustible 3R4F reference cigarettes.
2018-03-08 | E-MTAB-5697 | biostudies-arrayexpress
Project description:Cigarette smoking is the leading cause of lung cancer worldwide. Carcinogens in smoke produced during the combustion of cigarette tobacco are responsible for airway epithelial changes underlying lung carcinogenesis. Reduction of harmful constituents by heating rather than combusting tobacco would be a sound strategy to reduce the risk for lung cancer. In this study we characterized the functional and molecular changes during long-term treatment of human bronchial epithelial cells with total particulate matter (TPM) from a new candidate modified risk tobacco product (cMRTP), the tobacco heated system 2.2 (THS2.2) in comparison with TPM from combustible 3R4F reference cigarettes.
2018-03-08 | E-MTAB-6119 | biostudies-arrayexpress
Project description:The expanding scale and nature of rice fraud in the global food system has caused major economic and human health concerns. Herein, an untargeted metabolomics approach based on the UHPLC-Q-Orbitrap-HRMS system was utilized for the discrimination between authentic and commercial Sengcu rice, a local specialty cultivated by terraced farming in northern Vietnam. A total of 8398 positive and 5250 negative mode compounds were introduced to multivariate statistical analyses for the construction of classification models. The first two principal components explaining 52% of the total variance in both datasets exhibited distinguished clusters of authentic against commercial Sengcu rice. Partial least squares-discriminant analysis models were optimized to obtain the optimal number of retained components, the optimal number of variables retained in each component and the best prediction distance type for model evaluation. One component containing five positive (DMG, RSA, RCA, PAL and BOSe) and six negative mode variables (PXP, RXP, TDHP, ISS, MXP and RGB) was sufficient to discriminate between authentic and commercial Sengcu rice. The classification error rate was less than 1.1310-4, as determined from repeated k-fold cross validation. These putative signature metabolites clearly separated authentic and commercial Sengcu rice in the hierarchical clustering models. In addition, the isolated metabolites also reflected the cultivation practices of terraced farming of authentic Sengcu rice. Overall, we have proposed an effective method for the identification of key metabolites from fingerprinting metabolomics, and it could serve as a fundamental approach for other in-depth food authentication studies.
2022-05-08 | ST002169 | MetabolomicsWorkbench
Project description:Cell adaptation to high salinity levels implicates the modification of different cellular, physiological and molecular mechanisms. However, studies about the cellular mechanisms that are implicated in salt adaptation are scarce in the literature. Tobacco BY-2 cell cultures are very homogeneous and are characterized by a continuous cell grow and high proliferation rate. These features make these cells a good model system. For this study, we have obtained a stable tobacco cell line adapted to grow at 250 mM of NaCl. To obtain a general view of this process, we have followed a microarray technique. Gene expression was analyzed using the Affymetrix microarray technique. We have used a microarray designed by Edwards et al. (2010) in tobacco (Total probes: 43768).
2012-11-28 | GSE42562 | GEO