Project description:Schistosome foxA is enriched in the parasites' stem/progenitor cells and the esophageal gland, an anterior digestive organ required for their survival. However, the molecular makeup of the esophageal gland remains unclear. Here, we took a comparative transcriptomics approach using foxA knockdown adult parasites. Differential expression analysis reveals 37 genes commonly downregulated in foxA knockdown males and females, most of which show high enrichment in the esophageal gland.
Project description:In the present study we compare the secretome from S. mansoni adult males and females using a label free quantitative proteomic approach
Project description:Schistosoma mansoni is a dioecious species, that is, it has two differentiated sexes. Interestingly, this sexual species evolved from a hermaphrodite ancestor. Indeed, most Platyhelminthes are hermaphrodites. Here we characterize the microRNAs of S. mansoni and quantify their differential expression between males and females.
Project description:Schistosoma mansoni is a dioecious species, that is, it has two differentiated sexes. Interestingly, this sexual species evolved from a hermaphrodite ancestor. Indeed, most Platyhelminthes are hermaphrodites. Here we characterize the microRNAs of S. mansoni and quantify their differential expression between males and females. Mice were infected with Schistosoma mansoni 1-2 weeks prior to dissection. RNA from two independent samples were extracted and sequenced with Illumina MiSeq technology and AB SOLiD 4 technology. Reads were mapped to the reference genome and microRNA detected and analyzed.
Project description:Transcriptional profiling using two subsequent developmental stages of Schistosoma mansoni (Egg vs. Miracidium; Cercaria vs. 7-days-old Schistosomulum; 7-days-old Schistosomulum vs. Adult worms