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ABSTRACT:
PROVIDER: PRJNA1168574 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Atriplex nummularia microbiome Caatinga Biome, Petrolina, PE, Brazil
| PRJNA1168509 | ENA
Project description:This study examined the functional response of a host (zebrafish) to implantation of a conspecific or allospecific (goldfish) gastrointestinal (GIT) microbiome followed by diet manipulation and the repercussions of these manipulations on host GIT physiology. Implantation of a native zebrafish biome successfully reintroduced wildtype (WT) communities with the exception of several rare, phylogenetically distant species. Implantation of a foreign goldfish biome created communities that were distinct from WT, suggesting that the seeding community created substantial differences from the native host communities. A mismatched ?natural? diet and an implanted allospecific biome enriched for rarer and more phylogenetically diverse bacteria. Transcriptional changes within the GIT clustered in relationship to biome treatments, mirroring clustering of biome implants. Implantation of an allospecific biome along with an altered diet markedly down-regulated approximately 70% of the transcripts involved in cholesterol biosynthesis, while tissue content analysis revealed an increase in total tissue cholesterol. Furthermore, transcripts involved in lipogenesis pathways were significantly downregulated and correlated with a striking decrease in intestinal lipase activity driven by both biome and diet. Glucose-6P dehydrogenase (G6PD) activities increased during dietary manipulations regardless of biome, while the allospecific biome down-regulated transcripts involved in gluconeogenesis and altered glucokinase (GK) and hexokinase (HK) activities regardless of diet. However, growth rates did not reveal an impact of these responses. Adult zebrafish are unable to reform proportional representation within bacterial communities following transplantation of an allospecific biome resulting in transcriptional and enzymatic alterations for lipid and carbohydrate metabolism that did not affect overall animal homeostasis.
2018-08-30 | GSE118496 | GEO
Project description:The central part of Brazil, consisting mostly of the Cerrado Biome, is considered to be the new frontier for increasing Brazilian wheat production. However, rainfed wheat production in that area must cope with drought stress. In order to better understand the drought response, we analyzed the mRNA profiling under drought in roots and leaves of the cultivar MGS1 Aliança (a well-adapted cultivar to the Cerrado). We identified 4,422 candidate genes in roots and leaves.
2016-11-29 | GSE81833 | GEO
Project description:Elucidating the genetic control of development of C3 and C4 photosynthesis. Atriplex rosea (C4) and Atriplex prostrata (C3) were studied along a leaf developmental gradient to compare development between C3 and C4. C3 Atriplex prostrata x C4 Atriplex rosea F1 hybrid were studied along the same developmental gradient and will aid in identifying regulatory elements involved in C3 and C4 leaf development.
2023-01-01 | GSE89920 | GEO
Project description:Elucidating the genetic control of C3 and C4 photosynthesis. Atriplex rosea (C4) and Atriplex prostrata (C3) were at maturity to compare expression between C3 and C4 in leaves, stems, and roots. Their F1 hybrid leaf was studied at maturity and will aid in identifying regulatory elements involved in C3 and C4 leaf development. Two C3 Atriplex prostrata x C4 Atriplex rosea F3 hybrids (F3003 and F3036) were sequenced at a mature leaf stage.
2023-01-01 | GSE90100 | GEO
Project description:Ziziphus nummularia is well distributed in the Thar desert encompassing India and Pakistan. It survives in regions with an average annual rainfall lesser than 100 mm. Transcriptome profiling was done in Z. nummularia genotype CIAHZN-J under control and drought-stress at -0.3MPa. Among the unique transcripts identified, 283 were found to be downregulated and 554 upregulated in comparison to control. The important down-regulated transcripts identified were: LRR receptor like serine/threonine protein kinases, oligo peptide transporter OPT family, myrcene synthase, LRR protein kinase family isoform1, ATP binding cassette transporter, phytochrome kinase, Pectin estrase inhibitor like-35, auxin efflux facilitator isoform 1, multidrug resistance protein, pectin methyl esterase 3 and ABC transporter B family. Similarly some of the upregulated transcripts are: Raffinose synthase family protein, wall associated receptor kinase like, acyl transferase like protein, cadmium/zinc transporting ATPase 3 like, sucrose synthase 6-like, cytochrome P-450 like, phosphatase 2C family protein and TT12-2 MATE transporter. This study will be very useful to identify novel genes that may be involved in drought tolerance of Z. nummularia.
2021-03-31 | GSE69728 | GEO
Project description:Polyethylene pollutions are considered inert in nature and adversely affect the entire ecosystem. Larvae of greater wax moths (Galleria mellonella) have the ability to masticate and potentially biodegrade polyethylene films at elevated rates. The wax moth has been thought to metabolize PE independently of gut flora, however, the role of the microbiome is poorly understood and degradation by the wax moth might be involved. To determine whether the salivary glands of the wax moth were potentially involved in the PE degradation, it was investigated how surface changes of polyethylene were affected by mastication and consumption. Formation of pitting and degradation intermediates, including carbonyl groups, indicated that salivary glands could assist in polyethylene metabolism. We investigated the biochemical effect of exposure to PE on the composition of the salivary gland proteome. The expression of salivary proteins was found to be affected by PE exposure. The proteins that were significantly affected by the exposure to PE revealed that the wax moth is undergoing general changes in energy levels, and that enzymatic pathways associated with fatty acid beta oxidation were induced during PE consumption.
2020-03-19 | PXD016834 | Pride