Project description:Elucidating the genetic control of development of C3 and C4 photosynthesis. Atriplex rosea (C4) and Atriplex prostrata (C3) were studied along a leaf developmental gradient to compare development between C3 and C4. C3 Atriplex prostrata x C4 Atriplex rosea F1 hybrid were studied along the same developmental gradient and will aid in identifying regulatory elements involved in C3 and C4 leaf development.
Project description:Elucidating the genetic control of C3 and C4 photosynthesis. Atriplex rosea (C4) and Atriplex prostrata (C3) were at maturity to compare expression between C3 and C4 in leaves, stems, and roots. Their F1 hybrid leaf was studied at maturity and will aid in identifying regulatory elements involved in C3 and C4 leaf development. Two C3 Atriplex prostrata x C4 Atriplex rosea F3 hybrids (F3003 and F3036) were sequenced at a mature leaf stage.
Project description:Ziziphus nummularia is well distributed in the Thar desert encompassing India and Pakistan. It survives in regions with an average annual rainfall lesser than 100 mm. Transcriptome profiling was done in Z. nummularia genotype CIAHZN-J under control and drought-stress at -0.3MPa. Among the unique transcripts identified, 283 were found to be downregulated and 554 upregulated in comparison to control. The important down-regulated transcripts identified were: LRR receptor like serine/threonine protein kinases, oligo peptide transporter OPT family, myrcene synthase, LRR protein kinase family isoform1, ATP binding cassette transporter, phytochrome kinase, Pectin estrase inhibitor like-35, auxin efflux facilitator isoform 1, multidrug resistance protein, pectin methyl esterase 3 and ABC transporter B family. Similarly some of the upregulated transcripts are: Raffinose synthase family protein, wall associated receptor kinase like, acyl transferase like protein, cadmium/zinc transporting ATPase 3 like, sucrose synthase 6-like, cytochrome P-450 like, phosphatase 2C family protein and TT12-2 MATE transporter. This study will be very useful to identify novel genes that may be involved in drought tolerance of Z. nummularia.
