Project description:Samples were obtained from the dorsal skin of frogs after a mild electrical stimuli. They were freeze-dried and resuspended in water: acetonitrile (7:3) with 0.1% (v/v) trifluoroacetic acid (TFA). The aim of the analyses was to identified secondary metabolites in particular from the Boana pulchella group. After isolation and characterization of two novel macrocyclic acylcarnitines (MACH) by a combination of LC-MS/MS, RMN and GC/MS we also putatively identified other substances from the same series.
Project description:Samples were obtained from the dorsal skin of frogs after a mild electrical stimuli. They were freeze-dried and resuspended in water: acetonitrile (7:3) with 0.1% (v/v) trifluoroacetic acid (TFA). The aim of the analyses was to identified secondary metabolites in particular from the Boana pulchella group. After isolation and characterization of two novel macrocyclic acylcarnitines (MACH) by a combination of LC-MS/MS, RMN and GC/MS we also putatively identified other substances from the same series.
Project description:The DNA isolated from 44 either frozen or FFPE Neuroendocrine Neoplasm (NEN) was analysed by NGS, to identify genes more likely to be subject to sequence variations among 523 cancer-related ones.
Project description:Plasma DNA from 558 malignancies, 263 benign and borderline tumors and 367 healthy control samples were collected and subjected to random short-gun whole genome sequencing.
Project description:This study aims to investigate the DNA methylation patterns at transcription factor binding regions and their evolutionary conservation with respect to binding activity divergence. We combined newly generated bisulfite-sequencing experiments in livers of five mammals (human, macaque, mouse, rat and dog) and matched publicly available ChIP-sequencing data for five transcription factors (CEBPA, HNF4a, CTCF, ONECUT1 and FOXA1). To study the chromatin contexts of TF binding subjected to distinct evolutionary pressures, we integrated publicly available active promoter, active enhancer and primed enhancer calls determined by profiling genome wide patterns of H3K27ac, H3K4me3 and H3K4me1.