Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression profile at single cell level of human control, MPHOSPH8-deficient and MORC2-deficient cerebral organoids at day 27.

ABSTRACT: Gene expression profile at single cell level of human control, MPHOSPH8-deficient and MORC2-deficient cerebral organoids at day 27.

PROVIDER: PRJNA1174146 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Gene expression profile at single cell level of human control, MPHOSPH8-deficient and MORC2-deficient cerebral organoids at day 27.

Project description:To translate our mouse data and to model the role of MPHOSPH8 and MORC2 in human brain development, we generated human three-dimensional (3D) cerebral organoids lacking MPHOSPH8 and MORC2. These cerebral organoids were compared to control organoids by single cell RNA sequencing (scRNA-seq) to analyze the cell composition and expression of clustered protocadherins.

2024-10-18 | GSE279721 | GEO

Assessing the genome-wide occupancy of MORC2

Project description:By comparing HeLa cells expressing V5 epitope-tagged MORC2 constructs, the goal of the experiment was to determine the genome-wide localisation of MORC2 in the presence or absence of the HUSH complex and upon inactivation of the CW domain. The occupancy of V5-MORC2 was also compared to that of endogenous TASOR.

2017-05-29 | GSE95451 | GEO

MORC2 promotes renal carcinogenesis via epigenetically downregulating DAPK1

Project description:epigenetic regulation plays a vital role in renal carcinogenesis. Here，we found epigenetic regulator MORC2 promoted carcinogenesis in kidney. Mechanistically, MORC2 functioned as oncogene via epigenetically silencing canonical tumor suppressor DAPK1 in kidney. Therefore, aMORC2—DAPK1 pro—oncogenic axis was identified in kindey.

2023-10-25 | GSE221129 | GEO

Assessing the impact of loss of MORC2 on the transcriptome

Project description:By comparing HeLa cells lacking MORC2 generated through CRISPR/Cas9-mediated gene disruption to wild-type HeLa cells, the goal of the experiment was to determine the effect of loss of MORC2 on the transcriptome.

2017-05-29 | GSE95452 | GEO

NGLY1-deficient cerebral organiods display abnormal neuronal differentiation

Project description:NGLY1-deficient cerebral organiods display abnormal neuronal differentiation

| PRJNA595973 | ENA

DNA methylation status determines the sensitivity of repeats to restriction by the HUSH-MORC2 complex

Project description:The human silencing hub (HUSH) complex binds to transcripts of LINE-1 retrotransposons (L1s) and other genomic repeats, recruiting MORC2 and other effectors to remodel chromatin. However, how HUSH and MORC2 operate alongside DNA methylation, a central epigenetic regulator of repeat transcription, remains poorly understood. Here we interrogate this relationship in human neural progenitor cells (hNPCs), a somatic model of brain development that tolerates removal of DNA methyltransferase DNMT1. Upon loss of MORC2 or HUSH subunit TASOR in hNPCs, L1s remain silenced by robust promoter methylation. However, genome demethylation and activation of evolutionarily-young L1s attracts MORC2 binding. Simultaneous depletion of DNMT1 and MORC2 causes massive accumulation of L1 transcripts. We identify the same mechanistic hierarchy at pericentromeric α-satellites and clustered protocadherin genes, repetitive elements important for chromosome structure and neurodevelopment respectively. Our data delineate the independent epigenetic control of repeats in somatic cells, with implications for understanding the vital functions of HUSH-MORC2 in hypomethylated contexts throughout human development.

2024-03-26 | GSE242143 | GEO

DNA methylation status determines the sensitivity of repeats to restriction by the HUSH-MORC2 complex [CUT&RUN]

2024-03-26 | GSE240384 | GEO

DNA methylation status determines the sensitivity of repeats to restriction by the HUSH-MORC2 complex [ONT cDNA sequencing]

2024-03-26 | GSE262483 | GEO

DNA methylation status determines the sensitivity of repeats to restriction by the HUSH-MORC2 complex [Bulk RNA-seq]

2024-03-26 | GSE240386 | GEO

Identifying genomic loci exhibiting chromatin decompaction upon loss of MORC2

Project description:We used the Differential Viral Integration (DIVA) technique to compare chromatin accessibility in wild-type versus MORC2 knockout HeLa cells.

2017-05-29 | GSE95457 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data