Project description:Here we report the effect of two comparative transcriptomics experiments effect on EDDS producing biosynthetic gene cluster. The goals of this study are to compare RNA sequencing derived transcriptomes of Amycolatopsis japonica at 24 hours in zinc treated and control culture conditions in combination with control and zur regulator deleted strains, in order to target other genes for the overexpression of EDDS cluster.

2022-02-26 | GSE197253 | GEO

BES performance of S. oneidensis pure- and co-culture

Project description:Bioelectrochemical systems employing mixed microbial communities as biocatalysts are gaining importance as potential renewable energy, bioremediation, or biosensing devices. While we are beginning to understand how individual microorganism species interact with an electrode as electron donor, not much is known about the interactions between different microbial species in a community. Here, we compare the bioelectrochemical performance of Shewanella oneidensis in a pure-culture and in a co-culture with the homolactic acid fermenter Lactococcus lactis. While S. oneidensis alone can only use lactate as electron donor for current production, the co-culture is able to convert glucose into current with a similar coulombic efficiency of approximately 17%, respectively. With (electro)-chemical analysis and transcription profiling, we found that the BES performance and S. oneidensis physiology were not significantly different whether grown as a pure- or co-culture. These co-culture experiments represent a first step in understanding microbial interactions in BES communities with the goal to design complex microbial communities, which specifically convert target substrates into electricity. Further, for the first time, we elucidated S. oneidensis gene expression with an electrode as the only electron acceptor. The expression pattern confirms many previous studies regarding the enzymatic requirements for electrode respiration, and it generates new hypotheses on the functions of proteins, which are so far not known to be involved in electrode respiration.

2010-12-01 | GSE20343 | GEO

Amycolatopsis sp. QT-25 breed:Amycolatopsis sp. | cultivar:Amycolatopsis sp.

Project description:Amycolatopsis sp. QT-25 breed:Amycolatopsis sp. | cultivar:Amycolatopsis sp. Genome sequencing and assembly

| PRJNA943068 | ENA

Amycolatopsis

Project description:Amycolatopsis sp. full genome sequencing

| PRJNA978978 | ENA

Methylophilus sp. 'Pure River'

Project description:Methylophilus sp. 'Pure River' Genome sequencing

| PRJNA1180274 | ENA

Proteomic analysis reveals metabolic versatility of Amycolatopsis sp. BX17: a strain native from milpa agroecosystem soil

Project description:Amycolatopsis sp. BX17 is an actinobacterium isolated from milpa soils that antagonizes the phytopathogenic fungus Fusarium graminearum. Metabolites secreted by the actinobacterium cultured in medium without glucose inhibited 100% the mycelial growth of F. graminearum RH1, while in medium supplemented with 20 g/L of glucose inhibition was 65%. With the aim of studying how the metabolism of strain BX17 is modulated by glucose, as the main carbon source, media with 0 and 20 g/L glucose were selected to analyze the intracellular proteins by quantitative label-free proteomic analysis.

2022-02-14 | PXD028644 | Pride

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