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Sample multiplexed scRNA-seq using Nanocoding method for sample barcode labeling

ABSTRACT: Sample multiplexed scRNA-seq using Nanocoding method for sample barcode labeling

PROVIDER: PRJNA1177468 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31110172.fastq.gz	Fastqsanger.gz
	SRR31110173.fastq.gz	Fastqsanger.gz
	SRR31110174.fastq.gz	Fastqsanger.gz
	SRR31110175.fastq.gz	Fastqsanger.gz
	SRR31110176.fastq.gz	Fastqsanger.gz

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Sample multiplexed scRNA-seq using Nanocoding method for sample barcode labeling

Project description:Sample multiplexed scRNA-seq is a promising strategy to overcome current barriers in high cost and potential technical variations by multiple scRNA-seq tests. In this study, we developed a highly efficienct novel sample barcode labeling method using DNA-encoded Lipid Nanoparticles ('Nanocoding') that could label cells with minimal dependence on their type or sample conditions. This method provids a roubust and general protocol for sample barcoding and multiplexing in scRNA-seq. We demonstrated the performance of Nanocoding through three scRNA-seq studies, which include: 1. mouse spleen cells mix (one dataset including 6 mouse spleen tissues samples); 2. HeLa-mouse Stromal Vascular Fraction(SVF) cells mix (one dataset containing mixed HeLa cell and SVF cell); 3. Aged-Young SVF cells mix (one dataset containing two SVF samples) tests. These studies showcased the biomodal distribution of barcode counts in different models with high signal-to-background ratio, as well as pan-cell labeling activity for efficient and accurate sample-multiplexing. By using Nanocoding, we profiled obsity and age related change in lipid metabolism associated genes or inflammatory related features, in various cell types from spleen or adipose tissues.

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