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RNA-Seq of nucleus pulposus cells with or without CircFUNDC1 overexpression

ABSTRACT: RNA-Seq of nucleus pulposus cells with or without CircFUNDC1 overexpression

PROVIDER: PRJNA1183814 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR31283426_1.fastq.gz	Fastqsanger.gz
	SRR31283426_2.fastq.gz	Fastqsanger.gz
	SRR31283427_1.fastq.gz	Fastqsanger.gz
	SRR31283427_2.fastq.gz	Fastqsanger.gz
	SRR31283428_1.fastq.gz	Fastqsanger.gz

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1 - 5 of 12

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RNA-Seq of nucleus pulposus cells with or without CircFUNDC1 overexpression

Project description:RNA-Seq was used to detect the dwonstream genes after CircFUNDC1 overexpression. Method: Three nucleus pulposus cell total RNA profiles of Ad-NC samples and Ad-CircFUNDC1 samples were analyzed.

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Expression data from synovium, bone marrow and nucleus pulposus

Project description:Synovial and bone marrow mesenchymal stem cells after intradiscally injection show regenerative effects of nucleus pulposus. Microarray analyses of rats were performed to investigate the closeness of the gene profiles between the nucleus pulposus cells and the synovial or bone marrow mesenchymal stem cells. To investigate interaction between synovial mesenchymal stem cells and nucleus pulposus cells, human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured, and species specific microarray were performed. Synovium of knee joints, bone marrow and nucleus pulposus were harvested from rat or human, and cells were isolated for RNA extraction and hybridization on Affymetrix microarrays. To compare the gene profiles each other, isolated cells were mono-cultured respectively, and human synovial mesenchymal stem cells and rat nucleus pulposus cells were co-cultured.

2010-10-11 | E-GEOD-24612 | biostudies-arrayexpress

MicroRNAs expression profiling of human nucleus pulposus cells: control vs. degeneration

Project description:MicroRNAs expression profiling of human nucleus pulposus cells derived from patients with disc degeneration in comparison with those derived from patients with scoliosis as control. Two-condition experiment: control nucleus pulposus cells vs. degenerative nucleus pulposus cells. Biological replicates: 3 control, 3 degenerated, independently harvested. Four replicates per array. The supplementary file 'GSE19943_fold_pvalue.txt' contains fold-changes and p-values.

2012-01-01 | E-GEOD-19943 | biostudies-arrayexpress

Expression data from bovine nucleus pulposus interverteral disc cells

Project description:Assessment of the putative differential gene expression profiles in high osmolality-treated bovine nucleus pulposus intervertebral disc cells for a short (5 h) and a long (24 h) time period. Identification of novel genes up- or down-regulated as an early or a late response to hyperosmotic stress. A 5 and 24 h-hyperosmotic treatment of nucleus pulposus cells led to transcriptional changes in >100 and 200 genes, respectively. Nucleus pulposus intervertebral disc cells were exposed to hyperosmotic stress for 5 and 24 h before RNA extraction and transcriptomics analysis. Three biological replicates were tested for each condition. Selected genes found to be differentially expressed were validated by RT-qPCR. Functional experiments were performed in order to assess the role of specific proteins encoded by genes found to be up-regulated in the osmo-reguatory response of intervertebral disc cells.

2015-06-19 | E-GEOD-59485 | biostudies-arrayexpress

RNA-seq of rat nucleus pulposus cells after stabilizing G-Quadruplexes

Project description:We report the RNA-seq data for rat nucleus pulposus cells after stabilizing G-Quadruplexes (G4). We sequenced rat nucleus pulposus cells treated with PDS, Braco-19 and Phen-DC3 separately and untreated with these G4 stabilizers, which could stabilize G4 structure in cells.

2022-11-02 | GSE194015 | GEO

human nucleus pulposus cell LC-MS/MS

Project description:To identify PPP1CA interactome in human nucleus pulposus cell

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human nucleus pulposus cell LC-MS/MS

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Expression data from synovium, bone marrow and nucleus pulposus

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Single cell sequencing of human nucleus pulposus

Project description:In order to discover the cell type in nucleus pulposus and find the cell type specific genetic change during intervertebral disc degeneration, we applied single cell RNA sequencing of nucleus pulposus tissue from degenerated and non-degenerated disc.

2023-12-31 | GSE153066 | GEO

Effect of recombinant Shh on gene expression of primary rat nucleus pulposus cells

Project description:In order to study the effect of recombinant Shh (rShh) on nucleus pulposus cells, primary nucleus pulposus cells from SD rats were obtained.Then, nucleus pulposus cells were treated with rShh at 200 ng/ml(dissolved in dmso) for 48h. Similarly, we treated nucleus pulposus cells with the same concentration of DMSO as the control group, and analyzed the data of 3 biological repeats of RNA-seq in each group for gene expression profile analysis.

2022-11-10 | GSE217505 | GEO

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