Project description:Gene expression was measured on the Affymetrix platform in iPrEC cell line transfected with wild-type USP2A, mutant USP2A, or empty vector.
Project description:Evaluating the identity of ubiquitinated and ubiquitin-associated proteins tandem co-purifying non-covalently with wild-type (17 glutamine) human Huntingtin fragment, amino acids 1-502 C-terminally tagged with the HA peptide epitope, basally expressed from the CMV promoter within vector pcDNA3.1 transiently transfected into striatal precursor cell line St14A, significantly elevated above cells transfected with empty pcDNA3.1 vector negative control, in cells co-transfected with human FLAG-ubiquitin.
Project description:Gene expression was measured on the Affymetrix platform in iPrEC cell line transfected with wild-type USP2A, mutant USP2A, or empty vector. Gene expression was measured in 3 distinct groups (cell transfected with wild-type USP2A, mutant USP2A, or the empty vector). For each group 5 biological replicates were obtained and gene expression was measured on the Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. Differential gene expression was obtained for all the 3 possible pair-wise comparisons (WT versus MUT, WT versus EV, and MUT versus EV).
Project description:Transcriptional profiling of Leishmania major parasites overexpressing LmSir2rp3 protein compared with wild-type cell line transfected with empty vector in normal growth conditions. Goal of this experiment was to evaluate the possible effect of LmSir2rp3 in the control of gene expression in this protozoan parasite that has a polycistronic transcription. Two-condition experiment, LmSir2rp3 overexpressor vs. Wild-type cell line bearing empty vector. Biological replicates, 3 LmSir2rp3 overexpressor replicates and 3 wild-type cell line bearing empty-vector
Project description:Transcriptional profiling of Leishmania major parasites overexpressing LmSir2rp3 protein compared with wild-type cell line transfected with empty vector in normal growth conditions. Goal of this experiment was to evaluate the possible effect of LmSir2rp3 in the control of gene expression in this protozoan parasite that has a polycistronic transcription.
Project description:Investigation of whole genome gene expression level changes in HT1080 fibrosarcoma cell line after transfection CRABP1 gene and R131A CRABP1 mutant (arginine-alanine substitution in a protein active site, protein lacks the ability to interact with retinoic acid), compared to HT1080 line transfected with empty pLXSN vector.
Project description:Empty vector, SRF-dM-VP16 (control construct lacking the SRF DNA binding domain), or SRF-VP16 were expressed in wild-type ES cells (E14wt), SRF heterozygotes (E99-/+) or two SRF ko cell lines (E81-/-,E100-/-). RNA was harvested 72h after transfection. Each condition was performed in duplicate, except E100-/- vector transfected. Keywords: ordered
Project description:Gene methylation profiling of immortalized human mesenchymal stem cells comparing HPV E6/E7-transfected MSCs cells with human telomerase reverse transcriptase (hTERT)- and HPV E6/E7-transfected MSCs. hTERT may increase gene methylation in MSCs. Goal was to determine the effects of different transfected genes on global gene methylation in MSCs.
