Project description:Arabidopsis ult1-3 mutant was shown to rescue the phenotype of LFYasEMF1 to that of WT. We performed microarray analysis of 7-d-old and 15-d-old seedlings of WT, LFYasEMF1, ult1-3 and LFYasEMF1/ult1-3 grown under short day to study the underlie mechanism of this phenotypic rescuing. Gene expression of 7 and 15 day old WT, LFYasEMF1, ult1-3 and LFYasEMF1/ult1-3 seedlings grown under short day condition were performed and compared.
Project description:Arabidopsis ult1-3 mutant was shown to rescue the phenotype of LFYasEMF1 to that of WT. We performed microarray analysis of 7-d-old and 15-d-old seedlings of WT, LFYasEMF1, ult1-3 and LFYasEMF1/ult1-3 grown under short day to study the underlie mechanism of this phenotypic rescuing.
Project description:Compared analysis of the transcriptomes of 12-day old seedlings from wild type Col-0 treated with NO for 15, 30 and 60 min vs untreated control seedlings. Samples were harvested 12 h after dawn of day 12 after sowing and seedlings were grown under long days (16 h light / 8 h darkness) photoperiodic conditions.
Project description:Arabidopsis thaliana, 14-day-old seedlings grow in 1/2 MS culture medium under short day condition, LC-MS. In this project, two different materials (One is in the wild type background, the other is in the mutant background.) were used to do CoIP experiments, and then mass spectrometry was carried out to compare the number of peptide segments obtained by the two materials.
Project description:10-day-old wild-type and homozygous transgenic Arabidopsis seedlings (overexpressing OsTOP6A3 and OsTOP6B) grown under normal growth conditions were used for total RNA isolation. The 5 micrograms of each total RNA sample was processed for microarray analysis according to Affymetrix protocol. Keywords: Arabidopsis, wild-type and transgenic seedlings, differential gene expression
Project description:We sequenced the poly(A)+ and poly(A)- samples of the roots and shoots from 10-day-old WT seedlings grown under P+ and P- condition. The WT plant refers to Columbia ecotype Arabidopsis seedlings. Each condition has two replicates. After total RNA extraction, ribosomal RNAs were removed using RiboMinus™ Plant Kit repeated two times. The poly(A)+ and poly(A)- constituent were separated with oligo(dT) magnetic beads (Oligotex mRNA Mini Kit, QIAGEN). Using a 2-fold change and a P-value <0.05 as the cut-off for selecting the differentially expressed transcripts, we globally identified novel noncoding lncRNAs.
Project description:10-day-old wild-type and homozygous transgenic Arabidopsis seedlings (overexpressing rice topoisomerase 6 subunit A1; OsTOP6A1) grown under normal growth conditions were used for total RNA isolation. The 5 micrograms of each total RNA sample was processed for microarray analysis according to Affymetrix protocol. Keywords: Arabidopsis, wild-type and transgenic seedlings, differential gene expression
Project description:Liquid grown, seven day old wild type Arabidopsis seedlings and seedlings carrying a cDNA encoding the ABA INSENSITIVE GROWTH 1 (ABIG1) gene under the control of an estrogen inducible promoter were treated with estrogen for 0, 60 and 120 minutes at which point seedlings were flash frozen and mRNA was extracted.