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Single-Cell RNA Sequencing unveils dynamic transcriptional profiles during the process of donkey spermatogenesis and maturation

ABSTRACT: Single-Cell RNA Sequencing unveils dynamic transcriptional profiles during the process of donkey spermatogenesis and maturation

PROVIDER: PRJNA1190115 | ENA |

REPOSITORIES: ENA

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Single-Cell RNA Sequencing unveils dynamic transcriptional profiles during the process of donkey spermatogenesis and maturation

Project description:With the increasing demand for donkey production, there has been a growing focus on the breeding of donkeys. However, our current understanding of the mechanisms underlying spermatogenesis and maturation in donkeys during reproduction remains limited.In this study, we constructed a single-cell RNA dataset to study the single-cell landscape of donkey spermatogenesis and maturation. This method allows us to analyze the cell composition in testicular and epididymal tissue, providing insights into the changes that occur during donkey spermatogenesis and maturation. In addition, different gene expression signatures associated with various spermatogenic cell types were found

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Zearalenone exposure enhanced the expression of tumorigenesis genes in donkey granulosa cells via the PTEN/PI3K/AKT signaling pathway

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