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Histone H3.3 regulates dynamic chromatin states during spermatogenesis

ABSTRACT: We used H3f3b-/- testes to analyze the impact of H3f3b derived H3.3 on spermatogenesis. Analysis of Histone H3 (Lys 4) tri-methylation in H3f3b WT/WT and H3f3b -/- testes tissue.

ORGANISM(S): Mus musculus

SUBMITTER: Paul Knoepfler

PROVIDER: E-GEOD-60207 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Histone H3.3 regulates dynamic chromatin states during spermatogenesis.

Yuen Benjamin T K BT Bush Kelly M KM Barrilleaux Bonnie L BL Cotterman Rebecca R Knoepfler Paul S PS

Development (Cambridge, England) 20140819 18

The histone variant H3.3 is involved in diverse biological processes, including development, transcriptional memory and transcriptional reprogramming, as well as diseases, including most notably malignant brain tumors. Recently, we developed a knockout mouse model for the H3f3b gene, one of two genes encoding H3.3. Here, we show that targeted disruption of H3f3b results in a number of phenotypic abnormalities, including a reduction in H3.3 histone levels, leading to male infertility, as well as ...[more]

PMID: 25142466

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