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Comparison of CRISPR-Cas9 genome-edited JEG-3 TAZ/WWTR1 knockout and wild type choriocarcinoma cells

ABSTRACT: Comparison of CRISPR-Cas9 genome-edited JEG-3 TAZ/WWTR1 knockout and wild type choriocarcinoma cells

PROVIDER: PRJNA1190490 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR31508612.fastq.gz	Fastqsanger.gz
	SRR31508613.fastq.gz	Fastqsanger.gz
	SRR31508614.fastq.gz	Fastqsanger.gz
	SRR31508615.fastq.gz	Fastqsanger.gz
	SRR31508616.fastq.gz	Fastqsanger.gz

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Comparison of CRISPR-Cas9 genome-edited JEG-3 TAZ/WWTR1 knockout and wild type choriocarcinoma cells

Project description:The transcriptional co-activator TAZ/WWTR1 plays a central role in the Hippo signaling pathway and acts as crucial mediator in maintaining organ size and tissue homeostasis. Changes in its activity can lead to diseases, including cancer, due to uncontrolled cell growth or aberrant cellular behavior. The aim of this study was to identify gene signatures that are specifically regulated by TAZ in human trophoblast cells. This was achieved by comparing JEG-3 wild-type and TAZ knockout choriocarcinoma cells, generated with CRISPR-Cas9 technology.

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Comparison of CRISPR-Cas9 genome-edited JEG-3 YAP knockout and wild type choriocarcinoma cells.

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Project description:The transcriptional co-activator TAZ (WWTR1) plays a crucial role in regulating gene expression related to cell proliferation, differentiation, and tissue homeostasis, particularly within the Hippo signaling pathway. Since TAZ does not directly bind to DNA, it largely relies on its protein-protein interaction network to perform its functions, necessitating a detailed characterization of the TAZ interactome. To achieve this, a GFP-Nanotrap-based affinity purification approach combined with LC-MS/MS protein identification was employed to document a comprehensive list of both known and novel components of the TAZ interactome in myogenic cells.

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