Project description:Drought represents a major constraint on maize production worldwide. Understanding the genetic basis for natural variation in drought tolerance of maize may facilitate efforts to improve this trait in cultivated germplasm. Here, using a genome-wide association study, we show that a miniature inverted-repeat transposable element (MITE) inserted in the promoter of a NAC gene (ZmNAC111) is significantly associated with natural variation in maize drought tolerance. For maize RNA-seq analysis, pooled tissues from three, eight-day-old maize seedlings were collected from transgenic and wild-type plants, prior to or after 2-hour dehydration, to conduct the RNA-seq analysis.
Project description:Transcriptional profiling of 4 maize varieties comparing genetic root response under control temperature conditions with genetic root response under low temperature conditions
Project description:In this study a transcriptomic approach (RNA-sequencing) was utilized to elucidate molecular responses of maize (Zea mays L.) primary roots of the inbred line B73 to water deficit to gain a better understanding of the mechanisms underlying drought tolerance. Kernels of the maize inbred line B73 were germinated in paper rolls soaked with distilled water until seedlings had a primary root length of 2 to 4 cm. For mild and severe water deficit conditions, seedlings were transferred to PEG8000 solution with water potentials of -0.2 MPa and -0.8 MPa, respectively. Water deficit treatment was applied for 6 h and 24 h. Each treatment was performed in four biological replicates each consisting of 10 roots.
Project description:Drought stress is the main factor restricting maize yield. Poly-γ-glutamic acid (γ-PGA) could significantly improve the drought resistance and yield of many crops. However, its high production costs and unclear long-term impact on soil ecology limit its large-scale application. In this study, genes (PgsA, B, C) that participate in γ-PGA synthesis were cloned from Bacillus amyloliquefaciens and transformed into maize for the first time. Under drought stress, transgenic maize significantly increased the ear length, ear weight and grain weight by 50% compared to the control, whereas the above yield characteristics increased by 2.33, 13.06, 19.34 and 18.36%, respectively under normal growth conditions. γ-PGA was mainly expressed in the mesophyll cells of maize leaf rosette structure and improved drought resistance and yield by protecting and increasing the expression of genes for the photosynthetic and carbon fixation. This study is an important exploration for maize drought stress molecular breeding and building resource-saving agriculture.
Project description:Development of crop varieties with high nitrogen use efficiency (NUE) is crucial for minimizing N loss, reducing environmental pollution and decreasing input cost. Maize is one of the most important crops cultivated worldwide and its productivity is closely linked to the amount of fertilizer used. A survey of the transcriptomes of shoot and root tissues of a maize hybrid line and its two parental inbred lines grown under sufficient and limiting N conditions by mRNA-Seq has been conducted to have a better understanding of how different maize genotypes respond to N limitation.
Project description:Conventional cellular transcriptome profiling (RNA-seq) data reports “steady-state” transcript abundance rather than actual transcription rates in the nucleus. It also lacks sensitivity to detect unstable and low-abundance RNA species. We generated these maize (Zea mays L.) B73 total cellular RNA samples, extracted directly from frozen 1-mm root tips, as a parallel experiment to compare to two other types of RNA extracted from isolated nuclei.
Project description:These maize (Zea mays L.) B73 cellular RNA samples were prepared from seedlings treated in 2 h water and extracted cellular RNA directly from frozen 1-mm root tips. They are parallel experiment to compare to two other types of RNA extracted from isolated nuclei.
Project description:Analysis of the maize alternative splicing landscape, including transcript discovery and quantification during development and drought
Project description:We report the generation of RNA-seq and ribosome profiling data on 14-day maize seedling before and after drought stress treatment. We performed RNA-seq and ribosome profiling on both well-watered (WW) and drought-stressed (DS) maize seedlings. Both data types had two biological replicates. Note: Samples in SRA were assigned the same sample accession. This is incorrect as there are different samples, hence “Source Name” was replaced with new values. Comment[ENA_SAMPLE] contains the original SRA sample accessions.