Project description:Previous studies from our lab have characterized a group of splicing factors called SR proteins that function to identify intron/exon borders in regulating metabolic homeostasis in the Drosophila fat body. Decreasing the function of one SR protein, 9G8, causes an increase in triglyceride storage; however, the function of 9G8 in other metabolic tissues like the fly intestine is not known. Decreasing 9G8 in the Drosophila intestine results in the accumulation of triglycerides; however, the 9G8 target genes responsible for this phenotype are unknown. To address this question, we performed RNA sequencing on Drosophila intestines with 9G8 levels reduced by RNAi.
Project description:Previous studies from our lab have characterized a group of splicing factors called SR proteins that function to identify intron/exon borders in regulating metabolic homeostasis in the Drosophila fat body. Decreasing the function of one SR protein, 9G8, causes an increase in triglyceride storage; however, the full complement of 9G8 target genes that regulate metabolism is unknown. To address this question, we performed RNA sequencing on Drosophila fat bodies with 9G8 levels reduced by RNAi.
Project description:Deep Sequencing of mRNA from the Drosophila melanogaster S2-DRSC cells that have been RNAi depleted of mRNAs encoding RNA binding proteins. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf Analysis of Poly(A)+ RNA from S2-DRSC Cells depleted of mRNAs encoding RNA binding proteins
Project description:Deep Sequencing of mRNA from the Drosophila melanogaster S2-DRSC cells that have been RNAi depleted of mRNAs encoding RNA binding proteins. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
Project description:Mutations in Drosophila Pngl lead to structural and functional abnormalities in the larval intestine. Here we report using RNA-sequencing of Drosophila larval intestines to determine the effects of loss of Pngl on the transcriptome in this tissue.
Project description:Profiling of changes in steady state RNA levels upon RNAi-mediated knockdown of the nucleosome remodeling ATPase ISWI in Drosophila SL2 cells. Quantitation of the corresponding proteomes has been performed to describe transcriptome-proteome relations. The analysis indicates a limited correlation of the two expression steps. Keywords: RNAi-mediated knockdown
Project description:5 day RNAi treatment to knockdown Enigma, CG9006, a Drosophila mitochondrial protein with homology to acyl-CoA dehydrogenases. Keywords: Genetic modification