Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Quercetin diminishes scleral ER stress and protein misfolding: High throughput transcriptome analysis in form-deprivation myopia of guinea pigs

ABSTRACT: Quercetin diminishes scleral ER stress and protein misfolding: High throughput transcriptome analysis in form-deprivation myopia of guinea pigs

PROVIDER: PRJNA1205399 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Quercetin diminishes scleral ER stress and protein misfolding: High throughput transcriptome analysis in form-deprivation myopia of guinea pigs

Project description:This study aims to verify the involvement of scleral endoplasmic reticulum stress (ERS) in form-deprived myopia (FDM) model of guinea pigs, investigate the therapeutic effects of quercetin (Qcn) on FDM as well as explore the underlying mechanisms in human scleral fibroblast (HSF).

2025-01-02 | GSE285669 | GEO

Proteomic study of the retina of guinea pigs with form-deprivation myopia

Project description:Myopia has become the major cause of visual impairment worldwide. However, its retina-related pathogenesis remains unclear. In recent years, proteomics has become a high-throughput tool to explain biological mechanisms. In this study, we examined the retinas of guinea pigs with form deprivation myopia using 4D label-free proteomics and found disorders of retinal metabolism in experimental myopia.

2023-07-12 | PXD043226 | Pride

Proteomics analysis of guinea pig retina at the early stage of myopia

Project description:This is a combined SWATH database of early myopic guinea pig retina. The retinal tissues were divided into lens induced myopia 4 days group (4-day LIM) and control group; 5 individual animals (10 retinas) were included. The potential biomarkers and underlying biochemical pathways during early myopia could be investigated using SWATH based proteomics approach.

2021-05-13 | PXD025003 | Pride

Guinea pig myopia model ocular posterior pole

Project description:Proteomic analyses of the ocular posterior pole tissues at 6 weeks after induction of form-deprived myopia and lens-induced myopia models in guinea pigs.

2022-11-01 | PXD037872 |

Widespread Involvement of Acetylation in the Retinal Metabolism of Form-Deprivation Myopia in Guinea Pigs

Project description:Myopia has become the major cause of visual impairment worldwide. Although the pathogenesis of myopia remains controversial, proteomics studies suggest that dysregulation of retinal metabolism is potentially involved in the pathology of myopia. Lysine acetylation of proteins plays a key role in regulating cellular metabolism, but little is known about its role in the form-deprived myopic retina. In this study, we performed acetylation proteomic analysis of the retinas of form-deprived myopic guinea pigs and found downregulated levels of acetylation of metabolism-critical enzymes in the retina. As the first report on retinal acetylation in myopic eyes, this study provides a reliable basis for further studies on retinal acetylation in myopic eyes

2023-07-12 | PXD043196 | Pride

Single-cell RNA sequencing in formative deprivation myopia guinea pigs

Project description:Single-cell RNA sequencing in formative deprivation myopia guinea pigs

| PRJNA1212750 | ENA

Single-cell RNA sequencing in formative deprivation myopia guinea pigs

Project description:This is a single-cell RNA sequencing data on the primary visual cortex of formative-deprived myopic guinea pigs.

2025-01-31 | GSE287437 | GEO

Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia

Project description:Myopia has become the major cause of visual impairment worldwide. Although the pathogenesis of myopia remains controversial, proteomics studies suggest that dysregulation of retinal metabolism is potentially involved in the pathology of myopia. Lysine lactylation of proteins plays a key role in regulating cellular metabolism, but little is known about its role in the form-deprived myopic retina. In this study, we performed lactylation proteomic analysis of the retinas of form-deprived myopic guinea pigs and found downregulated levels of lactylation of metabolism-critical enzymes in the retina. As the first report on retinal lactylation in myopic eyes, this study provides a reliable basis for further studies on retinal lactylation in myopic eyes.

2024-10-17 | PXD046495 | Pride

Diurnal Retinal and Choroidal Gene Expression Patterns Distinguish Myopia Progression from Myopia Onset

Project description:Following laboratory and clinical findings implicating circadian biology in the pathogenesis of myopia (nearsightedness), we examined gene expression in two crucial tissues controlling post-natal refractive development, the retina and choroid. Inducing unilateral visual form deprivation myopia in young chicks, a widely studied and validated model, we isolated retinal and choroidal tissues every 4 hours over a single day from myopic and contralateral control eyes during a period time when myopia progresses rapidly.

2024-07-01 | GSE261232 | GEO

MiR-204-5p may Regulate Oxidative Stress in Myopia and Myopia-Related Retinopathy

Project description:Introduction: The mechanisms underlying myopia and myopia-related retinopathy remain not fully understood. We proposed to examine the function and underlying mechanisms of miR-204-5p in myopia development. Methods: The miR-204-5p expression level was assessed in the vitreous humor (VH) of a cohort consisting of 11 patients with high myopia (HM) and 16 control patients undergoing retinal surgery. The functional implications of miR-204-5p in ARPE-19 cells were assessed, encompassing cell aggressiveness. Thioredoxin-interacting protein (TXNIP) was found as a possible target of miR-204-5p through mRNA sequencing, and its interaction with miR-204-5p was confirmed employing luciferase assay and western blotting. Furthermore, the miR-204-5p function in regulating oxidative stress was examined by measuring reactive oxygen species (ROS) accumulation. Results: miR-204-5p was found to be significantly reduced in the VH of HM patients. Overexpression of miR-204-5p suppressed cell proliferation, migration, invasion, and apoptosis in ARPE-19 cells. The bioinformatics analysis demonstrated that miR-204-5p can modulate the genes associated with pathways relevant to myopia, including glycosaminoglycan (GAG) degradation, lysosome, and TGF−beta signaling pathway. The direct targeting of miR-204-5p on TXNIP has been confirmed through validation, and its downregulation mediated the miR-204-5p impacts on ARPE-19 cells. Moreover, miR-204-5p overexpression significantly reduced ROS accumulation by targeting TXNIP. Conclusion: Our findings revealed the possible contribution of the miR-204-5p/TXNIP axis in myopia development by regulating oxidative stress, which may provide new targets and novel therapeutic strategies to combat this prevalent and debilitating condition.

2024-01-26 | GSE253960 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data