Project description:Differentially regulated miRNA candidates in H37Rv infected THP-1 cells were analysed with respect to uninfected THP-1 reference samples. THP-1 cells are monocytes differentiated to macrophages after treatment with PMA for 48 hrs. Total RNA was isolated from infected THP-1 cells after 24 hrs of infection, cDNA was synthesized for TLDA real time PCR reaction using TaqMan MicroRNA Reverse Transcription kit and Megaplex Human Pool A and Pool B stem loop RT primers (version 3.0) as per manufacturer’s protocol. Further real time reaction was performed on QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems) by using cDNA (without pre-amplification) on TLDA card A and card B (version 3.0).
Project description:To compare gene expression changes induced by infection with Mycobacterium tuberculosis (Mtb) with changes induced by purified Mtb products, we infected THP-1 cells with Mtb strain H37Rv or treated with purified Mtb products, then performed RNAseq.
Project description:Perturbation of host physiology by intracellular Mycobacterium tuberculosis (H37Rv) can be reflected by changes in gene expression pattern of host genes. Total RNA was isolated from PMA differentiated uninfected cells or cells infected with H37Rv for 16, 48 or 90 hours and gene expression profile was obtained. There are in all 8 samples, two replcates of each 4. Two samples, namely 1D,1E (replicates of one) are control (PMA differentiated Thp-1 cells Uninfected controls). Cells were infected with H37Rv at an MOI of 1:10 and samples were collected at 16 hours, 48 hours and 90 hours post-infection.
Project description:Transcriptional profiling of Mtb H37Rv infected into THP-1 macrophage cell line and treated with 100 µM vitamin C (vit C) for 96 hours and 144 hours, compared to gene expression profile of untreated bacteria post-infection.
Project description:In this study, we employed high throughput sequencing (RNA-Seq) to investigate the gene expression profiles in THP-1 (monocyte cells )before and post-infection of VACV. we conducted RNA-Seq and analyzed differentially expressed genes in THP-1 cells with and without VAVC infection, and then performed the enrichment analysis, function annotation, and STRING network analysis. 4976 genes with significant differential expression (2416 were up-regulated and 2380 were down-regulated). Further KEGG enrichment analysis suggested that 27 significant signaling pathways, including metabolism, immune inflammation regulation, cell function regulation, cancer and myocardial disease, pathogen infection, were affected by VACV infection. Gene Ontology (GO) functional annotation indicated that these genes were mainly enriched on cell level regulation, immunological processes, protein levels, and signal transduction pathway. The STRING online database analysis showed that JUN,CHUK,IL1B,PYCARD protein were higher in the infected THP-1 cells.
