Project description:Gene expression was compared between hTERT-RPE1 cells and hTERT-RPE1 cells stably overexpressing mouse MFRP with an N-terminal GFP fusion.
Project description:Gene expression was compared between hTERT-RPE1 cells and hTERT-RPE1 cells stably overexpressing mouse MFRP with an N-terminal GFP fusion. RNA was prepared from hTERT-RPE1 cells overexpressing GFP-MFRP and control cells. Both conditions were done in triplicate. Affymetrix GeneChip Human Genome U133Plus2.0 arrays were used to interrogate changes in gene expression. Image data were quantified with Affymetrix Expression Console Software and normalized with Robust Multichip Analysis.
Project description:Transcriptional regulator YAP is activated in multiple human cancers and plays critical roles in tumor initiation, progression, metastasis, and drug resistance. However, therapeutic targeting of the Hippo-YAP pathway has been challenging due to its low druggability and limited knowledge of YAP regulation in cancer. Kinome siRNA library screening using an oncogenic YAP activation model identified the serine/threonine kinase MK5 as a positive regulator of YAP. To explore transcriptome signatures of MK5 activity, we performed RNA sequencing analysis after MK5 knockdown in LATS1/2-null RPE1 cells.
Project description:The impact of depleting SAF-A (HNRNPU) on the genome-wide replication timing program in human hTERT-RPE1 cells was assessed by a single-cell replication timing analysis.
Project description:We used HeLa cells and RPE1 cells after knockdown (KD) of USP39 using siRNAs. We compared differential expression and alternative splicing between Control and KD cells.