Project description:To investigate changes in SCN photic responsiveness after exposure to an ultradian light cycle (T7 cycle; alternating 3.5-hour periods of light and darkness), we performed RNA-sequencing on isolated SCN samples obtained from T24 and T7 light cycle-housed mice that received a light pulse during their active phases. Our results indicate that the SCN becomes refractory to light induction of immediate-early gene expression after exposure to the T7 light cycle.
Project description:Mar1 deletion and RNA enrichment in Cryptococcus neoformans: pilot data for a high-throughput sequencing course. The goal of this project was to generate pilot data in preparation for a summer course on high-throughput sequencing where participants prepared their own RNA-Seq libraries and analyzed the resulting data. This pilot experiment addressed two questions: 1. Does this experimental system (Cryptococcus neoformans H99 wildtype and mar1 deletion mutant grown in YPD and tissue culture media) provide a good dataset for course participants to analyze. 2. Which rRNA depletion method is best to use in the wetlab component of the course. This data was generated in preparation for the intensive summer course on high-throughput sequencing, funded by NIH grant 5R25EB023928-03 "A hands-on, integrative next-generation sequencing course: design, experiment, and analysis".
Project description:Following on from a pilot study to investigate the diversity of the coccolithoviruses, a second small scale microarray experiment was performed in order to provide genomic content information to aid the sequencing of a third coccolithovirus genome, EhV-99B1.
Project description:This transcription profiling time course experiment was conducted in order to analyze the cellular response of the plasmid-free expression system E.coli BL21(DE3)::TN7<T7 -SOD> to high level expression of recombinant human super-oxide-dismutase (SOD).Three biological replicates were generated by using a carbon limited exponential fed-batch cultivation similar to industrial setups for large scale production. For induction of the system a single pulse of isopropyl-beta-D-galactoside (IPTG) yielding in a fully induced system is applied one doubling past feed start.
Project description:Populations of engineered metabolite-producing microorganisms are prone to evolutionary production declines during industrial-scale cultivations. In this study, we develop a synthetic product addiction system in E coli that addicts mevalonic acid production cells to mevalonic acid. Through experimentally simuluated long-term fermentation, we investigate how product-addicted organisms remain stable and avoid formation of genetic subpopulations of fit, non-producing cells.
