Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Candidate transmission survival genome of Mycobacterium tuberculosis-CRISPRi Screen III

PROVIDER: PRJNA1209129 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR31962800_1.fastq.gz	Fastqsanger.gz
	SRR31962800_2.fastq.gz	Fastqsanger.gz
	SRR31962801_1.fastq.gz	Fastqsanger.gz
	SRR31962801_2.fastq.gz	Fastqsanger.gz
	SRR31962802_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 250

Similar Datasets

Project description:Candidate transmission survival genome of Mycobacterium tuberculosis-CRISPRi Screen I

| PRJNA1208037 | ENA

Project description:Candidate transmission survival genome of Mycobacterium tuberculosis-CRISPRi Screen II

| PRJNA1208562 | ENA

Project description:Candidate transmission survival genome of Mycobacterium tuberculosis - RNAseq data

| PRJNA1140188 | ENA

DIA/SWATH of the whole cell proteome of Mycobacterium tuberculosis

Project description:Untargeted DIA/SWATH of the whole cell proteome of Mycobacterium tuberculosis CRISPRi mutants

2023-12-21 | PXD041697 | Pride

Project description:Large scale chemical-genetic CRISPRi screen in Mycobacterium tuberculosis

| PRJNA738381 | ENA

Mycobacterium tuberculosis efpA CRISPRi knock-down transcriptional profile - induced efpA knock-down strain vs non-induced condition

Project description:In Mycobacterium tuberculosis, the efflux pump, EfpA, plays an essential but uncharacterised role in its physiology and antibiotic tolerance. In this study, an ATc-inducible CRISPR interference (CRISPRi) system was introduced into M. tuberculosis to knock-down the expression of efpA and determine any subsequent effects on the organism. As part of the study, microarray experiments were performed to determine any changes in gene expression caused by efpA repression M. tuberculosis by comparing the transcriptomic profile between ATc-induced efpA knock-down cultures to that of efpA knock-down cultures without ATc-induction.

2024-06-19 | E-MTAB-13755 | biostudies-arrayexpress

Expression Analysis of WR99210-treated Mycobacterium tuberculosis H37Rv

2012-09-18 | GSE40917 | GEO

Cellular active small molecule inhibitors of Mycobacterium tuberculosis by NMR fragment screen

Project description:Cellular active small molecule inhibitors of Mycobacterium tuberculosis by NMR fragment screen.

2015-01-01 | GSE17424 | GEO

Expression Analysis of WR99210-treated Mycobacterium tuberculosis H37Rv

Project description:Investigation of whole genome gene expression level changes in Mycobacterium tuberculosis treated with the DHFR inhibitor WR99210, compared to untreated cells. The antimycobacterial properties of WR99210 are further described in Gerum, A., Ulmer, J., Jacobus, D., Jensen, N., Sherman, D., and C. Sibley. 2002. Novel Saccharomyces cerevisiae screen identifies WR99210 analogues that inhibit Mycobacterium tuberculosis dihydrofolate reductase. Antimicrob Agents Chemother 46(11):3362-3369 [PMID:12384337] A nine chip experiment in which total RNA was collected from H37Rv after treatment with 100 uM WR99210 for 0, 4, and 24 hours. Three separate cultures/biological replicates were interrogated for both treated and untreated cultures. Labeled cDNA was hybridized to NimbleGen spotted oligo tiling arrays covering both strands of the M. tuberculosis genome at ~200 bp intervals between 60-mer probes.

2012-09-18 | E-GEOD-40917 | biostudies-arrayexpress

The involvement of Mycobacterium type III-A CRISPR-Cas system in oxidative stress and intracellular fitness

Project description:Previous studies suggest that type I and type II CRISPR-Cas systems were employed to evade host immunity by targeting interference of bacteria’s own genes. Although Mycobacterium bovis (M. bovis) and Mycobacterium tuberculosis (M. tuberculosis) possess integrated type III-A CRISPR-Cas system, its role in mycobacteria remains obscure. Here, we observed that seven cas genes (csm2~6, cas10, cas6) were significantly upregulated under oxidative stress treatment. To explore the function of type III-A CRSIRP-Cas system, Total CRISPR system (TCR) mutant strain was generated in M. bovis Bacille Calmette-Guérin (BCG). Deletion of TCR results in increased sensitivity in response to H2O2 and reduced cell envelope integrity. By using RNA-Seq, 590 differential expression genes were found in mutant for TCR, of which 220 genes were upregulated and 370 genes were downregulated, indicating an important role of TCR in controlling gene expression in mycobacteria. Consistently, disrupting TCR results in poor intracellular survival in vivo and in vitro. Moreover, we showed for the first time that TCR contributed to the regulation of regulatory T cell population, supporting a role of TCR in modulating host immunity. These observations demonstrate that type III-A CRISPR-Cas system as an important factor for intracellular survival and host immunoregulation in mycobacteria, may be a potential target for therapy.

2021-02-05 | GSE166137 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data