Project description:The aim of the experiment is to look at transcriptional changes in null mutants of APS kinase. There are four APS kinase isoforms in Arabidopsis, we are interested in the double mutant APK1/APK2, created by crossing two single salk lines. The transcriptomes of WT (Col-0) plants and the double knockout (ab) will be compared. The plants were grown under controlled environment conditions of 10h day, 14h night, 22 degrees centigrade, 60% humidity for five weeks. 10 whole rosettes were pooled for each replicate, there are three replicates for each genotype. Keywords: Expression profilling by array
Project description:The aim of the experiment is to look at transcriptional changes in null mutants of APS kinase. There are four APS kinase isoforms in Arabidopsis, we are interested in the double mutant APK1/APK2, created by crossing two single salk lines. The transcriptomes of WT (Col-0) plants and the double knockout (ab) will be compared. The plants were grown under controlled environment conditions of 10h day, 14h night, 22 degrees centigrade, 60% humidity for five weeks. 10 whole rosettes were pooled for each replicate, there are three replicates for each genotype. Keywords: Expression profiling by array 6 samples were used in this experiment
Project description:By knocking out genes encoding specific arogenate dehydrase (ADT) enzymes in Arabidopsis, variable reductions in lignin can be achieved. To understand how ADT composition affects plant phenotypes and biomolecular systems, we successfully constructed single and multiple ADT knockout (KO) mutants in Arabidopsis. Using these mutants, a multi-omics (metabolome, transcriptome and proteome) evaluation was conducted using GC- and LC-MS, RNA-Seq, and iTRAQ labeled LC-MS/MS technologies. Identifications include primary and secondary metabolites, transcripts, and proteins in leaf and stem samples taken at 4 weeks of age from 9 KO and wild-type (WT) lines.
