Project description:Transcriptional profiling of CD8 Tcm cells comparing untreated control cells to 16 hour norepinephrine treated cells. The goal was to examine the impact of norepinephrine treatment on CD8 Tcm cell global gene expression compared to untreated cells, and also to examine changes that occurred in resting cells and activated cells 24 and 72 hours after stimulation with CD3/28 beads. Two-conditional experiment: Control vs. NE treated CD8 Tcm cells. Biological replicates: 3 replicates for 0 hour and 24 hours, and 2 replicates for 72 hours. Each sample consisted of pooled RNA from 3 different donors.

2015-03-01 | E-GEOD-64635 | biostudies-arrayexpress

In vitro generation of CD8 Trm cells

Project description:In vitro generation of CD8 Trm cells

| PRJNA974072 | ENA

Murine CD8+ T cells: in vitro-derived vs. thymocytes

Project description:Transcriptional profiling of mouse in-vitro derived CD8+ cells (obtained after culturing BM-HSCs isolated from B6 mice) with OP9-DL1 cells for 35 days were compared to CD8+ thymocytes isolated from 4-5 weeks old B6 mice. Our goal was to determine similarities and differences in gene expression profile between in vitro-derived CD8+ cells and CD8+ cells isolated from the thymus. The procedure of in vitro differentiation of HSC using OP9-DL1 cocultures was previously described by: Schmitt, T. M., and J. C. Zuniga-Pflucker. 2002. Induction of T cell development from hematopoietic progenitor cells by delta-like-1 in vitro. Immunity 17:749-756 and Holmes, R., and J. C. Zuniga-Pflucker. 2009. The OP9-DL1 system: generation of T-lymphocytes from embryonic or hematopoietic stem cells in vitro. Cold Spring Harb Protoc 2009:pdb prot5156. Biological replicates: 2 different samples of in vitro-derived CD8+ T cells from day 35 were compared to 2 different samples of thymic CD8+ cells.

2012-09-05 | E-GEOD-37669 | biostudies-arrayexpress

Human CD8 T central memory (Tcm) cells: Control vs. norepinephrine (NE) treated

2015-03-01 | GSE64635 | GEO

RNA-seq of BATF-overexpressing CD8 T cells in vitro

Project description:RNA-seq of BATF-overexpressing CD8 T cells in vitro

| PRJNA516427 | ENA

Transcription profiling of mouse na´ve CD8+ T-cells vs. control mouse total RNA treated with IL-15 and/or IL21 vs controls to investigate regulation of CD8 cells

Project description:murine naive CD8+ T-cell samples for control or treated with 100ng/ml of IL-15 and/or IL-21 for 4 hours

2007-11-23 | E-GEOD-2059 | biostudies-arrayexpress