Ontology highlight
ABSTRACT:
PROVIDER: PRJNA1215929 | ENA |
REPOSITORIES: ENA
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Project description:Tire-wear particles (TWPs) are considered among the largest contributors of microplastics to the environment. They are subject to break-down due to environmental weathering, which allows for potentially toxic chemicals to be leached from and sorbed onto the particles. In this study, leachate generated from “weathered” and “un-weathered” TWPs were used for sublethal toxicity tests with Americamysis bahia.
2025-01-01 | GSE223584 | GEO
Project description:Rationale Microplastics are a pressing global concern and inhalation of microplastic fibers has been associated with interstitial and bronchial inflammation in flock workers. However, how microplastic fibers affect the lungs is unknown. Objectives Our aim was to assess the effects of 12x31 µm nylon 6,6 (nylon) and 15x52 µm polyethylene terephthalate (polyester) textile microplastic fibers on lung epithelial growth and differentiation. Methods We used human and murine alveolar and airway-type organoids as well as air-liquid interface cultures derived from primary lung epithelial progenitor cells and incubated these with either nylon or polyester fibers or nylon leachate. In addition, mice received one dose of nylon fibers or nylon leachate and 7 days later organoid-forming capacity of isolated epithelial cells was investigated. Results We observed that nylon microfibers, more than polyester, inhibited developing airway organoids and not established ones. This effect was mediated by components leaching from nylon. Epithelial cells isolated from mice exposed to nylon fibers or leachate, also formed fewer airway organoids, suggesting long-lasting effects of nylon components on epithelial cells. Part of these effects were recapitulated in human air-liquid interface cultures. Transcriptome analysis revealed upregulation of Hoxa5 post-exposure to nylon fibers. Inhibiting Hoxa5 protein during nylon exposure restored airway organoid formation, confirming Hoxa5's pivotal role in the effects of nylon. Conclusions These results suggest that components leaching from nylon 6,6 may especially harm developing airways and/or airways undergoing repair and we strongly encourage to characterize both hazard of and exposure to microplastic fibers in more detail.
2023-12-31 | GSE238065 | GEO
Project description:Tire-wear particle leachate toxicity to Americamysis bahia: analysis of sublethal and molecular effects
| PRJNA926920 | ENA
Project description:This dataset contains results for a semi-untargeted metabolomics study on fungal metabolite identification of the tire wear compounds acetanilide and hexamethoxymethylmelamine. High-resolution Orbitrap mass spectrometry was used along with Compound Discoverer (version 3.1) for downstream analysis. Further information is available in the readme file and related publication.
2022-02-22 | MSV000088889 | MassIVE
Project description:ngs2014_07_hevea-hevea_tpd-seq-RNAseq analysis of latex samples from healthy and Tapping Panel Dryness-affected trees.-Identification of Tapping Panel Dryness (TPD)-affected trees in a polyclonal trials grown under standard condition. Trees were tapped since November 2010 every 2 days. Latex yield and TPD occurrence were monitored as well as latex RNA samples were collected twice a year for further analysis. At the end of the experiment, gene expression in latex of healthy and TPD trees were compared.
2018-06-06 | GSE101568 | GEO
Project description:The Russian dandelion Taraxacum koksaghyz synthesizes considerable amounts of high molecular weight rubber in its roots. The cis-1,4-isoprene polymers are stored as rubber particle in the latex, the cytosol of specialized cells, called laticifers. The rubber transferase complex localizes at the particle surface and catalyzes the elongation of the cis-1,4-isoprene chains. The heteromeric complex is composed of an NgBR-like protein and a cis-prenyltransferase. In T. koksaghyz two NgBR homologous proteins, TkCPTL1 and TkCPTL2 were identified. Spatial expression analysis showed that TkCPTL1 is predominantly expressed at a high rate in latex, whereas TkCPTL2 exhibits low mRNA levels in all tissues. We investigated the functions of TkCPTL1&2 in two RNAi approaches. The down-regulation of TkCPTL2 showed no altered phenotype indicating a redundant function of the protein in dandelion. Knockdown of TkCPTL1 led to abolished poly(cis-1,4-isoprene) synthesis indicating that TkCPTL1 is essentially contributing to the formation of the rubber polymer. Simultaneously, levels of triterpenes and inulin in roots were significantly elevated. Analyses of latex from TkCPTL1-RNAi plants yielded a newly appearing latex fraction still containing particles, which seemed to be unaffected in structure compared to native rubber particles. Particle size, phospholipid composition and presence of small rubber particle proteins were determined for this purpose. We could show that the particles encapsulate triterpenes in a phospholipid shell that is stabilized by SRPPs. MS-based comparison of latex proteomes from TkCPTL1-RNAi plants and T. koksaghyz wildtypes enabled evidences for regulation mechanisms in several metabolic pathways associated to rubber biosynthesis.
2022-03-02 | PXD010953 | Pride
Project description:TIRE-seq (Turbocapture Integrated RNA Expression Sequencing) is a novel RNA sequencing method that integrates mRNA purification directly into library preparation, eliminating separate RNA extraction. Compared to traditional protocols like Primeseq, TIRE-seq demonstrates improved sequencing efficiency using crude cell lysates. We validated the method across three biological applications: analyzing transcriptional changes in stimulated human T cells, identifying genes driving murine dendritic cell differentiation, and examining temozolomide's dose-response effects on patient-derived neurospheres. TIRE-seq offers a streamlined, cost-effective approach for gene expression studies, reducing workflow complexity and minimizing sample loss.
2024-12-19 | GSE284395 | GEO