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Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development

ABSTRACT: Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development

PROVIDER: PRJNA1216537 | ENA |

REPOSITORIES: ENA

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Transcriptional changes of Tex10 in purified primordial gem cell-like cells (PGCLCs) at day2 and day6

Project description:Testis-specific transcript 10 (Tex10) is highly expressed in the testis, embryonic stem cells (ESCs), and primordial germ cells (PGCs). We previously generated a Tex10 knockout mouse model demonstrating its critical roles in ESC pluripotency and preimplantation development. Here, using conditional knockout mice and dTAG-degron ESCs, we show Tex10 is required for spermatogenesis and ESC-to-PGCLC differentiation. Specifically, Tex10-null spermatocytes arrest at metaphase I, compromising round spermatid formation. Tex10 depletion and overexpression compromise and enhance ESC-to-PGCLC differentiation, respectively. Mechanistically, bulk and single-cell RNA sequencing reveals that Tex10 depletion downregulates genes involved in pluripotency, PGC development, and spermatogenesis while upregulating genes promoting somatic programs. Chromatin occupancy study reveals that Tex10 binds to H3K4me3-marked promoters of Psmd3 and Psmd7, negative regulators of Wnt signaling, and activates their expression, thereby restraining Wnt signaling. Our study identifies Tex10 as a previously unappreciated factor in spermatogenesis and PGC development, offering potential therapeutic insights for treating male infertility.

2025-02-01 | GSE288151 | GEO

Tex10 in spermatogenesis

Project description:Tex10 in spermatogenesis

| PRJNA785665 | ENA

Tex10 in primordial gem cell-like cell (PGCLC) specification and spermatogenesis

Project description:Tex10 depletion compromises the PGCLC specification and spermatogenesis

2023-06-14 | GSE180800 | GEO

Tex10 in day2 embryonic bodies (EBs)

2025-02-01 | GSE288153 | GEO

Tex10 in spermatogenesis

Project description:Conditional Tex10 knockout compromises the spermatogenesis

2023-06-14 | GSE190072 | GEO

Transcriptional changes of spermatogenic cells by Tex10 deficiency

2025-02-01 | GSE288152 | GEO

Tex10 in primordial gem cell-like cell (PGCLC) specification and spermatogenesis

Project description:Tex10 in primordial gem cell-like cell (PGCLC) specification and spermatogenesis

| PRJNA749589 | ENA

Tex10 in PGCLC specification and spermatogenesis

Project description:Tex10 in PGCLC specification and spermatogenesis

| PRJNA749585 | ENA

Pluripotency factor Tex10 finetunes Wnt signaling for spermatogenesis and primordial germ cell development

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-02-01 | GSE288186 | GEO

Tex10 Coordinates Epigenetic Control of Super-Enhancer Activity for Pluripotency and Reprogramming [ChIP-Seq]

Project description:Super-enhancers (SEs) are large clusters of transcriptional enhancers that are co-occupied by multiple lineage specific transcription factors driving expression of genes that define cell identity. In embryonic stem cells (ESCs), SEs are highly enriched for Oct4, Sox2, and Nanog in the enhanceosome assembly and express enhancer RNAs (eRNAs). We sought to dissect the molecular control mechanism of SE activity and eRNA transcription for pluripotency and reprogramming. Starting from a protein interaction network surrounding Sox2, a key pluripotency and reprogramming factor that guides the ESC-specific enhanceosome assembly and orchestrates the hierarchical transcriptional activation during the final stage of reprogramming, we discovered Tex10 as a novel pluripotency factor that is evolutionally conserved and functionally significant in ESC self-renewal, early embryo development, and reprogramming. Tex10 is enriched at SEs in a Sox2-dependent manner and coordinates histone acetylation and DNA demethylation of SEs. Our study sheds new light on epigenetic control of SE activity for cell fate determination. Genome binding/occupancy profiling of Tex10 was performed in mouse embryonic stem cells by ChIP sequencing.

2015-05-07 | E-GEOD-66735 | biostudies-arrayexpress

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